酶联免疫吸附测定法（ELISA）和逆转录聚合酶链反应（RT-PCR）用于检测杏仁（扁桃）中李坏死环斑病毒和李属矮缩病毒的比较

Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis).

作者信息

Mekuria Genet, Ramesh Sunita A, Alberts Evita, Bertozzi Terry, Wirthensohn Michelle, Collins Graham, Sedgley Margaret

机构信息

School of Agriculture and Wine, The University of Adelaide, Waite Campus, PMB #1, Glen Osmond, SA 5064, Australia.

出版信息

J Virol Methods. 2003 Dec;114(1):65-9. doi: 10.1016/j.jviromet.2003.08.014.

DOI:10.1016/j.jviromet.2003.08.014

PMID:14599680

Abstract

A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.

摘要

已开发出一种基于逆转录聚合酶链反应（RT-PCR）的技术，用于同时检测杏仁中李坏死环斑病毒（PNRSV）和李矮缩病毒（PDV）的存在。本文介绍了一项为期3年的研究结果，该研究使用175份杏仁叶样本，比较了酶联免疫吸附测定（ELISA）和RT-PCR检测PNRSV和PDV的效果。发现多重RT-PCR比ELISA更灵敏，尤其是在随后进行巢式PCR检测PDV时。RT-PCR技术还有一个额外的优点，即可以在整个生长季节的任何时间对植物材料进行检测。