Sluyter Steven, Klis Sjaak F L, de Groot John C M J, Smoorenburg Guido F
Hearing Research Laboratories, Department of Otorhinolaryngology, University Medical Center Utrecht, Room G.02.531, P.O. Box 85.500, 3508 GA Utrecht, The Netherlands.
Hear Res. 2003 Nov;185(1-2):49-56. doi: 10.1016/s0378-5955(03)00260-0.
We have investigated whether or not cisplatin-induced depression of the endocochlear potential (EP), and its subsequent recovery, possesses a morphological correlate in the stria vascularis. Guinea pigs implanted with round window electrodes were treated daily with cisplatin (1.5 mg/kg/day) until the compound action potential showed a profound hearing loss (> or =40 dB at 8 kHz after 5-18 days). Animals were either sacrificed immediately after the shift in hearing threshold ('SHORT' group) or allowed to recover for > or =4 weeks and subsequently sacrificed ('LONG' group). Control animals ('CONTROL' group) were not treated with cisplatin. Using stereological methods we measured the total strial cross-sectional area together with the areas occupied by the different strial components: the marginal, intermediate and basal cells. The total strial cross-sectional area in the basal turn of the LONG group was found to be significantly smaller than that of the SHORT and the CONTROL groups, whereas the EP was normal in the LONG group (in comparison to the CONTROL group) and markedly decreased in the SHORT group. The smaller area in the LONG group was mainly due to a decrease in the area occupied by the intermediate cells and to a lesser extent to a decrease in the marginal cell area. The area occupied by the basal cells did not change. Thus, the marked decrease in EP after 5-18 days of cisplatin administration was not related to shrinkage of the stria vascularis. Moreover, 4 weeks later the EP showed full recovery, whereas the stria vascularis had shrunk markedly.
我们研究了顺铂引起的内淋巴电位（EP）降低及其随后的恢复，是否与血管纹的形态学变化相关。给植入圆窗电极的豚鼠每日注射顺铂（1.5mg/kg/天），直至复合动作电位显示出严重听力损失（5-18天后在8kHz处≥40dB）。动物在听力阈值改变后立即处死（“SHORT”组），或让其恢复≥4周后处死（“LONG”组）。对照动物（“CONTROL”组）未用顺铂治疗。我们采用体视学方法测量了血管纹的总横截面积以及不同血管纹成分（边缘细胞、中间细胞和基底细胞）所占的面积。发现LONG组蜗底的血管纹总横截面积明显小于SHORT组和CONTROL组，而LONG组的EP正常（与CONTROL组相比），SHORT组则明显降低。LONG组面积减小主要是由于中间细胞所占面积减少，边缘细胞面积减少程度较小。基底细胞所占面积没有变化。因此，给予顺铂5-18天后EP的显著降低与血管纹萎缩无关。此外，4周后EP显示完全恢复，而血管纹已明显萎缩。
