Gündoğdu Ramazan, Erkan Mustafa, Aydın Mesut, Sönmez Mehmet Fatih, Vural Alperen, Kökoğlu Kerem, Karabulut Derya, Şahin Mehmet İlhan
Department of Otolaryngology, Derince Tranining and Research Hospital, Kocaeli, Turkey.
Department of Otolaryngology, Head and Neck Surgery, Erciyes University School of Medicine, Kayseri, Turkey.
J Int Adv Otol. 2019 Aug;15(2):229-236. doi: 10.5152/iao.2019.5902.
This study aimed to evaluate the effect of quercetin on cochlear function and morphology, and its possible protective effect against acute cisplatin-induced ototoxicity in rats.
This prospective and controlled animal study was conducted on Wistar albino rats divided into four groups. Otoacoustic emission measures were performed three days after the first infiltration in Group 1 (saline), 2 (cisplatin), and 3 (quercetin). This interval was five days for Group 4 (cisplatin+quercetin). At the end of the study, the rats were decapitated with deep anesthesia, and histological changes in the cochleas were observed by light microscopy.
Group 2 (cisplatin) revealed significant differences between the first and second measures in all frequencies. When compared to other group, the difference of the changes in Group 2 statistically significantly decreased, especially in higher frequencies. Morphologically, there were no acute changes in Group 1 and Group 3. Outer hair cell loss and the degeneration of stria vascularis and spiral ganglion were observed in both Groups 2 and 4; the damages in the latter were lesser.
Quercetin does not have negative effect on cochlea, and it has protective effect on cisplatin-induced ototoxicity.
本研究旨在评估槲皮素对大鼠耳蜗功能和形态的影响，及其对顺铂诱导的急性耳毒性可能的保护作用。
本前瞻性对照动物研究在分为四组的Wistar白化大鼠上进行。第1组（生理盐水）、第2组（顺铂）和第3组（槲皮素）在首次注射后三天进行耳声发射测量。第4组（顺铂+槲皮素）的这个间隔为五天。研究结束时，大鼠在深度麻醉下断头，通过光学显微镜观察耳蜗的组织学变化。
第2组（顺铂）在所有频率的首次和第二次测量之间显示出显著差异。与其他组相比，第2组变化的差异在统计学上显著降低，尤其是在高频。形态学上，第1组和第3组没有急性变化。在第2组和第4组均观察到外毛细胞丢失以及血管纹和螺旋神经节的退化；后者的损伤较轻。
槲皮素对耳蜗没有负面影响，并且对顺铂诱导的耳毒性具有保护作用。
