Sun Jiu-Song, Zhang Tian-Yi, Zhang Qi-Rui, Xu Wei-Hua
Department of Molecular and Cell Biology, University of Science and Technology of China, Hefei, 230027, China.
Regul Pept. 2003 Nov 15;116(1-3):163-71. doi: 10.1016/j.regpep.2003.09.002.
Recent studies in Helicoverpa armigera report a novel role for diapause hormone (DH), pheromone biosynthesis activating neuropeptide (PBAN) and three other FXPRLamide neuropeptides secreted from suboesophageal ganglion (SG) in terminating pupal diapause. In the present paper, we investigated the role of these five FXPRLamide family neuropeptides on pupal development. Although removal of SG could not make nondiapause-destined pupae enter diapause-like status, it did make them eclose approximately 0.6-1.2 days later when compared with the controls. The results of competitive ELISAs showed a high level of FXPRLamide titer in the hemolymph of the SG-removed pupae and this may be due to the expression of the DH-PBAN gene in tissues other than SG. DH-PBAN mRNA and peptides were also detected in the thoracic ganglia (TGs) by RT-PCR and immunocytochemistry. The expression of DH-PBAN gene in the TGs of the SG-removed pupae is significantly higher than that in normal pupae by quantitative PCR and immunocytochemistry. Decerebration experiments proved that the decerebrated pupae could enter diapause-like status through down-regulation of FXPRLamide titer in hemolymph. Our studies confirm that the brain plays an important role in the determination of pupal development by regulating the synthesis and release of FXPRLamide neuropeptides in H. armigera. Thus, the function of FXPRLamide peptides in H. armigera is closely correlated with pupal development.
近期对棉铃虫的研究报告称，滞育激素（DH）、信息素生物合成激活神经肽（PBAN）以及从咽下神经节（SG）分泌的其他三种FXPRLamide神经肽在终止蛹滞育过程中发挥了新作用。在本文中，我们研究了这五种FXPRLamide家族神经肽对蛹发育的作用。虽然去除咽下神经节并不能使非滞育蛹进入类似滞育的状态，但与对照组相比，确实使它们羽化时间推迟了约0.6 - 1.2天。竞争性酶联免疫吸附测定结果显示，去除咽下神经节的蛹血淋巴中FXPRLamide滴度较高，这可能是由于咽下神经节以外的组织中DH - PBAN基因的表达。通过逆转录聚合酶链反应（RT - PCR）和免疫细胞化学也在胸神经节（TGs）中检测到了DH - PBAN mRNA和肽。通过定量PCR和免疫细胞化学检测发现，去除咽下神经节的蛹的胸神经节中DH - PBAN基因的表达明显高于正常蛹。去脑实验证明，去脑蛹可以通过下调血淋巴中FXPRLamide滴度进入类似滞育的状态。我们的研究证实，大脑通过调节棉铃虫中FXPRLamide神经肽的合成和释放，在蛹发育的决定中起着重要作用。因此，棉铃虫中FXPRLamide肽的功能与蛹发育密切相关。
