脂多糖对大鼠库普弗细胞中CD14和TLR4表达的影响。
The effect of lipopolysaccharides on the expression of CD14 and TLR4 in rat Kupffer cells.
作者信息
Feng Jun-Ming, Shi Jing-Quan, Liu You-Sheng
机构信息
Research Institute of Pathology, Southwestern Hospital, Third Military Medical University, Chongqing 400038, China.
出版信息
Hepatobiliary Pancreat Dis Int. 2003 May;2(2):265-9.
OBJECTIVE
To assess the effect of lipopolysaccharides (LPS) on the expression of CD14 and TLR4 in rat Kupffer cells (KCs).
METHODS
In rat KCs induced by LPS, the changes of CD14 and TLR4 expression were measured by RT-PCR and immunohistochemistry, and the expressions of TNF-alphamRNA, IL-6mRNA or the concentrations of TNF-alpha, IL-6 were estimated by in situ hybridization, radioimmunoassay, and others.
RESULTS
The expressions of CD14 and TLR4 in KCs induced by LPS were markedly increased in a dose-dependent manner (10 mg/L-1 microg/L) or in a time-dependent manner (0.5 h-24 h), with the peaked expression of CD14 at 3-6 hours. The expressions of CD14 and TLR4 in KCs stimulated by the active mediators from KCs which had been exposed to LPS for 1 hour were obviously increased.
CONCLUSIONS
There is a close relationship between LPS or the active mediators from KCs induced by LPS and the expressions of CD14, TLR4. It is implied that the increase of TLR4, CD14 expression may be induced by LPS within 1-3 hours, and further increase of TLR4, CD14 expression may be correlated with the cytokines produced by KCs.
目的
评估脂多糖（LPS）对大鼠库普弗细胞（KCs）中CD14和TLR4表达的影响。
方法
在LPS诱导的大鼠KCs中，通过逆转录聚合酶链反应（RT-PCR）和免疫组织化学检测CD14和TLR4表达的变化，并通过原位杂交、放射免疫测定等方法评估肿瘤坏死因子-α（TNF-α）mRNA、白细胞介素-6（IL-6）mRNA的表达或TNF-α、IL-6的浓度。
结果
LPS诱导的KCs中CD14和TLR4的表达以剂量依赖性方式（10 mg/L - 1 μg/L）或时间依赖性方式（0.5小时 - 24小时）显著增加，CD14在3 - 6小时表达达到峰值。用已暴露于LPS 1小时的KCs的活性介质刺激的KCs中CD14和TLR4的表达明显增加。
结论
LPS或LPS诱导的KCs的活性介质与CD14、TLR4的表达密切相关。这表明TLR4、CD14表达的增加可能在1 - 3小时内由LPS诱导，TLR4、CD14表达的进一步增加可能与KCs产生的细胞因子有关。
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