Arathi G, Sachdanandam P
Department of Medical Biochemistry, Dr. A.L.M. Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, India.
J Pharm Pharmacol. 2003 Sep;55(9):1283-90. doi: 10.1211/0022357021710.
Semecarpus anacardium Linn. of the family Anacardiaceae has many applications in the Ayurvedic and Siddha systems of medicine. We have evaluated the effect of S. anacardium nut milk extract on carbohydrate metabolizing enzymes and mitochondrial tricarboxylic acid cycle and respiratory enzymes in liver and kidney mitochondria of dimethyl benzanthracene-induced mammary carcinoma in Sprague-Dawley rats. Mammary carcinoma-bearing rats showed a significant rise in glycolytic enzymes (hexokinase, phosphoglucoisomerase and aldolase) and a simultaneous fall in gluconeogenic enzymes (glucose-6-phosphatase and fructose 1,6-diphosphatase). The activities of mitochondrial enzymes isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, NADH-dehydrogenase and cytochrome C oxidase were significantly lowered in mammary carcinoma-bearing rats when compared with control rats. S. anacardium nut extract administration to tumour-induced animals significantly lowered the glycolytic enzyme activities (hexokinase, phosphoglucoisomerase and aldolase) and there was a rise in gluconeogenic enzymes (glucose-6-phosphatase and fructose 1,6-diphosphatase), which indicated an antitumour and anticancer effect. Comparison of normal control rats and rats administered S. anacardium only as drug control animals showed no significant variations in enzyme activities. S. anacardium nut extract administration to dimethyl benzanthracene-tumour-induced animals significantly increased the activities of mitochondrial enzymes, thereby suggesting its role in mitochondrial energy production.
漆树科的印度没药在阿育吠陀和悉达医学体系中有多种应用。我们评估了印度没药坚果奶提取物对二甲基苯并蒽诱导的斯普拉格-道利大鼠乳腺癌肝脏和肾脏线粒体中碳水化合物代谢酶、线粒体三羧酸循环及呼吸酶的影响。患乳腺癌的大鼠糖酵解酶（己糖激酶、磷酸葡萄糖异构酶和醛缩酶）显著升高，同时糖异生酶（葡萄糖-6-磷酸酶和果糖1,6-二磷酸酶）下降。与对照大鼠相比，患乳腺癌大鼠线粒体酶异柠檬酸脱氢酶、α-酮戊二酸脱氢酶、琥珀酸脱氢酶、苹果酸脱氢酶、NADH脱氢酶和细胞色素C氧化酶的活性显著降低。给肿瘤诱导动物施用印度没药坚果提取物可显著降低糖酵解酶活性（己糖激酶、磷酸葡萄糖异构酶和醛缩酶），糖异生酶（葡萄糖-6-磷酸酶和果糖1,6-二磷酸酶）升高，这表明其具有抗肿瘤和抗癌作用。将正常对照大鼠与仅作为药物对照动物施用印度没药的大鼠进行比较，酶活性无显著差异。给二甲基苯并蒽诱导肿瘤的动物施用印度没药坚果提取物可显著增加线粒体酶的活性，从而表明其在线粒体能量产生中的作用。
