Premalatha B, Sujatha V, Sachdanandam P
Department of Medical Biochemistry, University of Madras, Taramani Campus, Chennai, 600113, India.
Pharmacol Res. 1997 Sep;36(3):187-92. doi: 10.1006/phrs.1997.0214.
The herbal remedy extended by Semecarpus anacardium nut extract against Aflatoxin B1 mediated hepatocellular carcinoma was established by studies on carbohydrate metabolizing enzymes. Since some definite correlation exists between tumour progression and the activities of glycolytic and gluconeogenic enzymes, assessment of alterations in their activity can be used as successful markers of diagnosis and prognosis. The present work compares the activities of glycolytic and gluconeogenic enzymes in hepatocellular carcinoma bearing rats with drug-treated animals. An overall increase in glycolytic enzymes namely hexokinase, phosphoglucoisomerase, and aldolase with a subsequent reduction in gluconeogenic enzymes, glucose-6-phosphatase and fructose-1,6-biphosphatase was observed in plasma and liver homogenates of hepatocellular carcinoma bearing rats. The administration of Semecarpus anacardium nut extract caused a significant decrease in the activity of glycolytic enzymes and an increase in gluconeogenic enzymes' activities to near normal values in drug-treated animals.
通过对碳水化合物代谢酶的研究，证实了印度没药坚果提取物对黄曲霉毒素B1介导的肝细胞癌具有草药治疗作用。由于肿瘤进展与糖酵解和糖异生酶的活性之间存在一定的相关性，因此评估它们活性的变化可作为诊断和预后的成功标志物。目前的工作比较了荷肝细胞癌大鼠与药物治疗动物中糖酵解和糖异生酶的活性。在荷肝细胞癌大鼠的血浆和肝匀浆中观察到糖酵解酶（即己糖激酶、磷酸葡萄糖异构酶和醛缩酶）总体增加，随后糖异生酶（葡萄糖-6-磷酸酶和果糖-1,6-二磷酸酶）减少。在药物治疗的动物中，给予印度没药坚果提取物导致糖酵解酶活性显著降低，糖异生酶活性增加至接近正常水平。
