Département des Sciences Animales, Faculté de l'Agriculture et de l'Alimentation, Université Laval, Québec, QC G1K 7P4, Canada.
J Ovarian Res. 2013 Sep 25;6(1):68. doi: 10.1186/1757-2215-6-68.
The use of gonadotropins to trigger oocyte maturation both in vivo and in vitro has provided precious and powerful knowledge that has significantly increased our understanding of the ovarian function. Moreover, the efficacy of most assisted reproductive technologies (ART) used in both humans and livestock species relies on gonadotropin input, mainly FSH and LH. Despite the significant progress achieved and the huge impact of gonadotropins, the exact molecular pathways of the two pituitary hormones, FSH and LH, still remain poorly understood. Moreover, these pathways may not be the same when moving from the in vivo to the in vitro context. This misunderstanding of the intricate synergy between these two hormones leads to a lack of consensus about their use mainly in vitro or in ovulation induction schedules in vivo. In order to optimize their use, additional work is thus required with a special focus on comparing the in vitro versus the in vivo effects. In this context, this overview will briefly summarize the downstream gene expression pathways induced by both FSH in vitro and LH in vivo in the cumulus compartment. Based on recent microarray comparative analysis, we are reporting that in vitro FSH stimulation on cumulus cells appears to achieve at least part of the gene expression activity after in vivo LH stimulation. We are then proposing that the in vitro FSH-response of cumulus cells have similitudes with the in vivo LH-response.
促性腺激素在体内和体外诱导卵母细胞成熟的应用提供了宝贵而有力的知识，极大地提高了我们对卵巢功能的理解。此外，大多数用于人类和家畜的辅助生殖技术（ART）的疗效都依赖于促性腺激素的输入，主要是 FSH 和 LH。尽管取得了重大进展，并对促性腺激素产生了巨大影响，但垂体激素 FSH 和 LH 的确切分子途径仍知之甚少。此外，当从体内环境转移到体外环境时，这些途径可能并不相同。这种对这两种激素之间复杂协同作用的误解导致了对它们的使用缺乏共识，主要是在体外或体内排卵诱导方案中。为了优化它们的使用，因此需要开展更多的工作，特别是要比较体外与体内的效果。在这种情况下，本文将简要总结 FSH 在体外和 LH 在体内诱导卵丘细胞中诱导的下游基因表达途径。基于最近的基因芯片比较分析，我们报告说，体外 FSH 对卵丘细胞的刺激似乎在体内 LH 刺激后至少部分地实现了基因表达活性。然后，我们提出体外 FSH 对卵丘细胞的反应与体内 LH 反应具有相似性。
