Poston S M, Palmer T J
J Gen Microbiol. 1977 Dec;103(2):235-42. doi: 10.1099/00221287-103-2-235.
Transduction of resistance from a multiply antibiotic-resistant strain of Staphylococcus epidermidis sub-group II was studied using the typing phage 108. The effect of increasing doses of ultraviolet radiation on the transducing phage was used to indicate the chromosomal or plasmid nature of the genes. Tetracycline and chloramphenicol resistance behaved as plasmid genes and streptomycin resistance as a chromosomal marker. It was also possible to transduce penicillin resistance (Pc) due to penicillinase production (bla+) using a low level of benzylpenicillin (0.03 microgram ml-1) for recovery. Approximately 10(-5) transductant colonies per phage input were obtained and ultraviolet kinetics indicated that Pc was plasmid carried. Pc transductants fell into two categories. In one group PC was stable as in the donor strain and transductants had the same phage sensitivity as the recipient. In the other, Pc was unstable at 37 degrees C and the instability was enhanced by growth at approximately 43.5 degrees C; these transductants also gained genes for restriction and modification of certain phages. Transductants that subsequently lost bla+ also lost the restriction and modification characters.
使用分型噬菌体108研究了来自表皮葡萄球菌II亚组多重耐药菌株的耐药性转导。利用紫外线辐射剂量增加对转导噬菌体的影响来表明基因的染色体或质粒性质。四环素和氯霉素耐药性表现为质粒基因，链霉素耐药性作为染色体标记。使用低水平的苄青霉素（0.03微克/毫升）进行回收时，也有可能转导由于产生青霉素酶（bla+）而导致的青霉素耐药性（Pc）。每个噬菌体输入大约获得10^(-5)个转导菌落，紫外线动力学表明Pc是由质粒携带的。Pc转导子分为两类。在一组中，Pc与供体菌株一样稳定，转导子与受体具有相同的噬菌体敏感性。在另一组中，Pc在37℃不稳定，在约43.5℃生长时不稳定性增强；这些转导子还获得了某些噬菌体的限制和修饰基因。随后失去bla+的转导子也失去了限制和修饰特性。
