Rosendorf L L, Kayser F H
J Bacteriol. 1974 Nov;120(2):679-86. doi: 10.1128/jb.120.2.679-686.1974.
A genetic analysis of a multiply antibiotic-resistant strain of Staphylococcus epidermidis was performed. Experiments designed to show reversion of organisms to antibiotic susceptibility, as well as studies of the influence of ultraviolet irradiation of phage on the transduction frequencies of the resistance markers, indicated that determinants of chloramphenicol (cml), tetracycline (tet), and neomycin (neo) resistance are present on separate plasmids, but the streptomycin marker is chromosomal. In 2 to 6% of tetracycline-resistant transductants, co-transduction of cml was also observed. By using CsCl-dye density gradients followed by neutral sucrose gradients, the plasmids carrying cml, tet, and neo could be isolated and their molecular weights could be determined. The tetracycline plasmid is shown to be incompatible with one of the cryptic plasmids of a recipient strain.
对一株多重耐药表皮葡萄球菌进行了基因分析。旨在显示细菌恢复对抗生素敏感性的实验，以及噬菌体紫外线照射对耐药标记转导频率影响的研究表明，氯霉素（cml）、四环素（tet）和新霉素（neo）耐药的决定因素存在于不同的质粒上，但链霉素标记是染色体的。在2%至6%的四环素耐药转导子中，也观察到了cml的共转导。通过使用CsCl-染料密度梯度随后进行中性蔗糖梯度，携带cml、tet和neo的质粒可以被分离出来并确定其分子量。四环素质粒与受体菌株的一种隐蔽质粒不相容。
