细胞内肌动蛋白作为体外肌成纤维细胞的标志物。

Intracellular actin as a marker for myofibroblasts in vitro.

作者信息

Foo I T, Naylor I L, Timmons M J, Trejdosiewicz L K

机构信息

Plastic Surgery and Burns Research Unit, Postgraduate School of Pharmacology, University of Bradford, United Kingdom.

出版信息

Lab Invest. 1992 Dec;67(6):727-33.

PMID:1460863

Abstract

BACKGROUND

Myofibroblasts are found in a wide variety of normal tissues and pathological conditions. It is suggested that myofibroblasts are derived from normal fibroblasts and share with smooth muscle cells the expression of actin microfilament bundles. The aim of this study was to establish if the myofibroblast phenotype from tissue expander capsules and Dupuytren's nodules could be distinguished from normal dermal fibroblasts by quantitation of intracellular actin and the ratio of polymerized (filamentous) actin to nonpolymerized (globular) actin.

EXPERIMENTAL DESIGN

Cell lines were established from six patients from each group. In addition to quantitation of intracellular actin, the cells were characterized by criteria of light microscopy, ultrastructure, actin immunofluorescence, and growth rates.

RESULTS

Dermal fibroblasts were the smallest and the most spindle-shaped but grew rapidly and had few actin microfilament bundles. By contrast, myofibroblasts from expander capsules were larger and more stellate, proliferated slowly, and had the most prominent microfilament arrays. Cells from Dupuytren's nodules were intermediate in phenotype. Substantial and significant differences in intracellular actin contents were found, ranging from 0.69 +/- 0.05 micrograms/10(4) cells in fibroblasts and 0.77 +/- 0.15 micrograms/10(4) cells for Dupuytren's nodule cells to 1.46 +/- 0.44 micrograms/10(4) cells in expander capsule myofibroblasts (p < 0.05). Similar findings were found with respect to ratios of fibroblast to globular actins, being 0.22 for fibroblasts and 0.38 for Dupuytren's nodule cells compared with 0.70 for expander capsule myofibroblasts (p < 0.01).

CONCLUSIONS

Measurement of intracellular actin contents and fibroblast:globular actin ratios offers a rapid, sensitive, and reliable technique for establishment of the myofibroblast phenotype and has considerable advantages over traditional ultrastructural approaches for the study of myofibroblast differentiation/regression and in vitro responses to experimental manipulation.

摘要

背景

肌成纤维细胞存在于多种正常组织和病理状态中。有研究表明，肌成纤维细胞起源于正常成纤维细胞，且与平滑肌细胞一样表达肌动蛋白微丝束。本研究的目的是通过定量细胞内肌动蛋白以及聚合型（丝状）肌动蛋白与非聚合型（球状）肌动蛋白的比例，来确定组织扩张器包膜和掌腱膜结节中的肌成纤维细胞表型是否可与正常真皮成纤维细胞相区分。

实验设计

从每组的6名患者中建立细胞系。除了定量细胞内肌动蛋白外，还通过光学显微镜、超微结构、肌动蛋白免疫荧光和生长速率等标准对细胞进行表征。

结果

真皮成纤维细胞最小，呈最细长的纺锤形，但生长迅速，肌动蛋白微丝束较少。相比之下，扩张器包膜的肌成纤维细胞更大，更呈星状，增殖缓慢，且具有最明显的微丝阵列。掌腱膜结节的细胞表型介于两者之间。细胞内肌动蛋白含量存在显著差异，成纤维细胞为0.69±0.05微克/10⁴细胞，掌腱膜结节细胞为0.77±0.15微克/10⁴细胞，扩张器包膜肌成纤维细胞为1.46±0.44微克/10⁴细胞（p<0.05）。关于成纤维细胞与球状肌动蛋白的比例也有类似发现，成纤维细胞为0.22，掌腱膜结节细胞为0.38，而扩张器包膜肌成纤维细胞为0.70（p<0.01）。