Tomasek J, Rayan G M
Department of Anatomical Sciences, University of Oklahoma Health Sciences Center, Oklahoma City, USA.
J Hand Surg Am. 1995 May;20(3):450-5. doi: 10.1016/S0363-5023(05)80105-4.
We studied 11 nodules from patients with Dupuytren's contracture to determine whether alpha-smooth muscle actin expression in Dupuytren's fibroblasts is related to the generation of contractile force. Tissue was placed into explant culture and fibroblast strains were obtained. The mean percent of cultured Dupuytren's fibroblasts expressing alpha-smooth muscle actin, as determined by immunofluorescence, was 14 +/- 8 and ranged from 1% to 26%. The ability of Dupuytren's fibroblasts to generate contractile force was determined by using a previously described collagen lattice contraction assay. We observed a significant positive correlation between the expression of alpha-smooth muscle actin and the generation of contractile force in cell strains of Dupuytren's fibroblasts. In addition, six fibroblast strains from palmar fascia of individuals undergoing carpal tunnel release were examined. In six strains of palmar fibroblasts the mean percent of cells expressing alpha-smooth muscle actin was 5 +/- 3 and ranged from 1% to 9%. Six Dupuytren's fibroblast strains, in which more than 15% of the cells expressed alpha-smooth muscle actin, were significantly more contractile than the palmar fibroblasts. These results suggest that Dupuytren's fibroblasts can acquire smooth muscle characteristics and that the acquisition of a smooth muscle-like phenotype correlates with increased contractility.
我们研究了11个来自掌腱膜挛缩症患者的结节，以确定掌腱膜成纤维细胞中α-平滑肌肌动蛋白的表达是否与收缩力的产生有关。将组织置于外植体培养中，获得成纤维细胞株。通过免疫荧光测定，培养的掌腱膜成纤维细胞中表达α-平滑肌肌动蛋白的平均百分比为14±8，范围为1%至26%。通过使用先前描述的胶原晶格收缩试验来测定掌腱膜成纤维细胞产生收缩力的能力。我们观察到在掌腱膜成纤维细胞株中，α-平滑肌肌动蛋白的表达与收缩力的产生之间存在显著的正相关。此外，对6例接受腕管松解术患者掌腱膜的成纤维细胞株进行了检测。在6株掌部成纤维细胞中，表达α-平滑肌肌动蛋白的细胞平均百分比为5±3，范围为1%至9%。6株超过15%的细胞表达α-平滑肌肌动蛋白的掌腱膜成纤维细胞株的收缩力明显高于掌部成纤维细胞。这些结果表明，掌腱膜成纤维细胞可获得平滑肌特性，且获得类似平滑肌的表型与收缩力增加相关。
