Park Mi-rim, Lee Eung-goo, Kim Yong-hwan, Jung Tae-sung, Shin Yong-seung, Shin Gee-wook, Cha Hui-guen, Kim Gon-sup
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, 900 Gazwa, Jinju 660-701, Korea.
J Vet Sci. 2003 Aug;4(2):143-9.
Protein identification by peptide mass fingerprinting using matrix-assisted laser desorption ionization time of fight (MALDI-TOF) mass spectrometry (MS) can analyze unambiguously identity of the spots from a 2-dimensional electrophoresis (2-DE) gel. This study developed a technique for 2-DE of Salmonella enterica serovar Enteritidis (S. enteritidis) by improving the dissolution conditions by 2-DE using a pH 4 - 7 immobilized pH gradient (IPG) strip. This report examines the protein components from the patterns of the S. enteritidis protein. The most abundant protein displayed a great number of clusters within the pH 4.5 - 7 range with a molecular mass ranging from 35-80 kDa. Some of these spots were identified as metabolic related enzymes. The protein fraction was also analyzed using an immobilized pH gradient strip. Different proteins were identified on the spot according to the elongation factors. In addition, this study showed that the 2-DE analysis of S. enteritidis provides useful information regarding the S. enteritidis proteome, and this approach might provide a strategy for identifying bacterial proteins using a proteome technology.
使用基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱（MS）通过肽质量指纹图谱进行蛋白质鉴定，可以明确分析二维电泳（2-DE）凝胶上斑点的身份。本研究通过使用pH 4-7固定化pH梯度（IPG）条改进二维电泳的溶解条件，开发了一种肠炎沙门氏菌肠炎血清型（肠炎沙门氏菌）二维电泳技术。本报告研究了肠炎沙门氏菌蛋白质模式中的蛋白质成分。最丰富的蛋白质在pH 4.5-7范围内显示出大量簇，分子量范围为35-80 kDa。其中一些斑点被鉴定为代谢相关酶。蛋白质部分也使用固定化pH梯度条进行分析。根据延伸因子在斑点上鉴定出不同的蛋白质。此外，本研究表明肠炎沙门氏菌的二维电泳分析提供了有关肠炎沙门氏菌蛋白质组的有用信息，并且这种方法可能为使用蛋白质组技术鉴定细菌蛋白质提供一种策略。
