Azazy A A, Nasher S T, Ishaq A, Bin-Muhanna M, Chance M L
Faculty of Medicine and Health Sciences, Sana'a University, PO Box 14460, Sana'a, Yemen.
Ann Trop Med Parasitol. 2003 Oct;97(7):689-95. doi: 10.1179/000349803225002481.
As part of an attempt to develop an effective method for the serodiagnosis of visceral leishmaniasis (VL), the sera from 43 Yemeni cases of the disease were screened both for antileishmanial IgG antibodies and for Leishmania-specific antigens. Indirect ELISA and capture ELISA were used to test each serum for the antibodies and antigens, respectively. Sera from patients with diseases other than leishmaniasis (29 for the antibody-detection assays and 42 for the antigen-detection) and from apparently healthy volunteers were also tested. For each type of assay, the threshold for seropositivity was set three standard deviations above the mean absorbance value for the sera from the healthy volunteers. Thirty-seven of the 43 VL sera were found positive for Leishmania-specific IgG antibodies and 37 were found positive for Leishmania-specific antigens. The sensitivities of the antibody- and antigen-detection assays were both therefore 86%. The overall specificity of the antibody-detection ELISA (67%) was, however, slightly higher than that of the capture-ELISA (64%).
作为开发内脏利什曼病（VL）血清学诊断有效方法的一部分，对43例也门VL患者的血清进行了抗利什曼原虫IgG抗体和利什曼原虫特异性抗原筛查。分别采用间接ELISA和捕获ELISA检测每份血清中的抗体和抗原。还检测了利什曼病以外疾病患者的血清（29份用于抗体检测试验，42份用于抗原检测）以及明显健康志愿者的血清。对于每种检测类型，血清阳性阈值设定为高于健康志愿者血清平均吸光度值3个标准差。43份VL血清中有37份利什曼原虫特异性IgG抗体检测呈阳性，37份利什曼原虫特异性抗原检测呈阳性。因此，抗体检测和抗原检测试验的灵敏度均为86%。然而，抗体检测ELISA的总体特异性（67%）略高于捕获ELISA（64%）。
