Martin S K, Thuita-Harun L, Adoyo-Adoyo M, Wasunna K M
Department of Entomology, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.
Ann Trop Med Parasitol. 1998 Jul;92(5):571-7. doi: 10.1080/00034989859267.
Leishmania donovani promastigotes were cultured in a protein-free medium for 3-5 days and the spent medium used to prepare antibody-detection ELISA plates. When the plates were used to test 29 Kenyan and 16 Nepalese patients with visceral leishmaniasis (VL; kala-azar), all the sera collected at diagnosis were found to have high levels of parasite-specific IgG. The levels of these antibodies dropped 6-12 months post-initiation of antileishmanial therapy in all but one of the patients. Although the levels in sera from 59% of the treated patients fell to those measured in sera from healthy controls, those in sera from 17% of the patients did not drop below those seen at diagnosis. The antigen used did not cross-react with sera from patients with parasitological diagnosis of malaria, filariasis, African trypanosomiasis or echinococcosis. Antibodies to antigens in the spent medium were detected, by western blot, in all the sera from Nepalese patients with VL. Promastigote-conditioned media could be the source of cheap antigen for the immunodiagnosis of leishmaniasis.
将杜氏利什曼原虫前鞭毛体在无蛋白培养基中培养3 - 5天，然后用培养后的培养基制备用于抗体检测的酶联免疫吸附测定（ELISA）板。当用这些板检测29名肯尼亚和16名尼泊尔内脏利什曼病（VL；黑热病）患者时，发现所有诊断时采集的血清中都有高水平的寄生虫特异性IgG。除一名患者外，所有患者在开始抗利什曼治疗后6 - 12个月，这些抗体水平下降。虽然59%接受治疗患者的血清抗体水平降至健康对照血清所测水平，但17%患者的血清抗体水平未降至诊断时所见水平以下。所用抗原与经寄生虫学诊断为疟疾、丝虫病、非洲锥虫病或棘球蚴病患者的血清无交叉反应。通过蛋白质印迹法在所有尼泊尔VL患者的血清中检测到了对培养后培养基中抗原的抗体。前鞭毛体条件培养基可能是用于利什曼病免疫诊断的廉价抗原来源。
