Choi I S, Yoo H S, Collisson E W
Department of Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Korea.
J Vet Sci. 2000 Dec;1(2):97-103.
It is known that CD28, a positive costimulatory receptor, plays a very important role in inducing the optimal stimulation of T lymphocytes. CTLA-4 (CD152), however, acts as a negative regulator in T lymphocyte activation. The effect of an feline immunodeficiency virus (FIV) infection on the expression of feline CD28 and CTLA-4 was studied with FIV-infected and uninfected peripheral blood mononuclear cells (PBMC) using a competitive PCR assay. The nature of CD28 and CTLA-4 expression was also examined with fresh and antigen-stimulated PBMC. FIV infection induced a lower expression of CD28, but a higher expression of CTLA-4 in the infected PBMC than in the uninfected PBMC. Relatively high levels of CD28 expression were demonstrated in both the fresh and the antigen-stimulated PBMC. The expression level of CTLA-4 in the freshly isolated PBMC was rather low, however, FIV antigen stimulation induced a relatively high expression of CTLA-4 in feline PBMC.
已知共刺激阳性受体CD28在诱导T淋巴细胞的最佳刺激中发挥着非常重要的作用。然而，细胞毒性T淋巴细胞相关抗原4（CTLA-4，CD152）在T淋巴细胞激活中起负调节作用。利用竞争性聚合酶链反应分析，对感染猫免疫缺陷病毒（FIV）和未感染的外周血单个核细胞（PBMC）中猫CD28和CTLA-4的表达情况进行了研究。还利用新鲜的和抗原刺激的PBMC检测了CD28和CTLA-4表达的性质。FIV感染导致感染的PBMC中CD28表达低于未感染的PBMC，但CTLA-4表达高于未感染的PBMC。新鲜的和抗原刺激的PBMC中均显示出相对较高水平的CD28表达。然而，新鲜分离的PBMC中CTLA-4的表达水平相当低，FIV抗原刺激诱导猫PBMC中CTLA-4相对较高的表达。
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