Omori M, Pu R, Tanabe T, Hou W, Coleman J K, Arai M, Yamamoto J K
Department of Pathobiology, College of Veterinary Medicine, University of Florida, P.O. Box 110880, Gainesville, FL 32611, USA.
Vaccine. 2004 Dec 2;23(3):386-98. doi: 10.1016/j.vaccine.2004.05.032.
Vaccine-induced T cell responses to FIV were assessed by measuring FIV-specific cytokine and cytotoxic-effector molecule production. A total of 22 cats at 10-12 weeks of age received either dual-subtype FIV vaccine (n=12), uninfected cell lysate (n=5) consisting of cells used to produce vaccine viruses, or no immunization (n=5). Significant increases in mRNA and protein production of T-helper 1 (Th1) cytokines (IL-2, IFNgamma), mRNA production of a cytotoxic-effector molecule (perforin), and lymphoproliferation response were observed in peripheral blood mononuclear cells (PBMC) from dual-subtype FIV-vaccinated cats after in vitro stimulation with inactivated FIV. In contrast, no statistically significant increase in FIV-stimulated mRNA production of Th2 cytokines (IL-4, IL-6) or other cytotoxic-effector molecules (TNFalpha, FasL) was observed in the PBMC from dual-subtype vaccinated cats. Moreover, no FIV-specific increases in the IFNgamma, IL-2, and perforin mRNA productions and in the IFNgamma bioactivity and lymphoproliferation responses were observed in the PBMC from cell-immunized cats. These observations suggest that IFNgamma induction, lymphoproliferation, and significant portion of IL-2 and perforin productions in the PBMC from dual-subtype vaccinated cats are clearly specific for viral antigens. Overall, dual-subtype FIV vaccine elicited strong Th1 response (IFN(, IL-2), which may contribute to the vaccine protection by enhancing the perforin-mediated cytotoxic-cell activity against FIV.
通过测量FIV特异性细胞因子和细胞毒性效应分子的产生来评估疫苗诱导的针对FIV的T细胞反应。总共22只10 - 12周龄的猫接受了双亚型FIV疫苗（n = 12）、由用于生产疫苗病毒的细胞组成的未感染细胞裂解物（n = 5）或未进行免疫（n = 5）。在用灭活FIV进行体外刺激后，在双亚型FIV疫苗接种猫的外周血单核细胞（PBMC）中观察到T辅助1（Th1）细胞因子（IL - 2、IFNγ）的mRNA和蛋白质产生、细胞毒性效应分子（穿孔素）的mRNA产生以及淋巴细胞增殖反应显著增加。相比之下，在双亚型疫苗接种猫的PBMC中，未观察到FIV刺激的Th2细胞因子（IL - 4、IL - 6）或其他细胞毒性效应分子（TNFα、FasL）的mRNA产生有统计学意义的增加。此外，在细胞免疫猫的PBMC中，未观察到IFNγ、IL - 2和穿孔素mRNA产生以及IFNγ生物活性和淋巴细胞增殖反应有FIV特异性增加。这些观察结果表明，双亚型疫苗接种猫的PBMC中IFNγ诱导、淋巴细胞增殖以及IL - 2和穿孔素产生的很大一部分显然对病毒抗原具有特异性。总体而言，双亚型FIV疫苗引发了强烈的Th1反应（IFNγ、IL - 2），这可能通过增强穿孔素介导的针对FIV的细胞毒性细胞活性来促进疫苗保护作用。
