一种用于诊断包虫病的试纸条染料免疫分析法的研发。
Development of a dipstick dye immunoassay for diagnosing hydatidosis.
作者信息
Sbihi Younes, Gil José R, Alvarez Pedro A, Orduña Antonio, Rodríguez-Torres Antonio, Osuna Antonio
机构信息
Instituto de Biotecnología, Campus Fuentenueva, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
出版信息
J Clin Lab Anal. 2003;17(6):219-22. doi: 10.1002/jcla.10097.
Abstract
We have developed a kit to diagnose hydatidosis, based on the detection of specific antibodies. This disease, caused by larvae of the cestode Echinococcus granulosus, is a zoonosis commonly found throughout the world. The diagnostic kit discriminates between positive and negative cases in a way that is easily interpreted, i.e., positive cases form a colored band on an inert substrate. The results show test sensitivity to be some 94.87% and specificity 85.71%, which is comparable to those of the ELISA technique. The high sensitivity and specificity of this test affords the great advantage of speed in diagnosing this parasitosis. No other equipment or procedure is required.
摘要
我们研发了一种基于特定抗体检测来诊断包虫病的试剂盒。这种由绦虫细粒棘球绦虫幼虫引起的疾病是一种在全球普遍存在的人畜共患病。该诊断试剂盒以易于解读的方式区分阳性和阴性病例，即阳性病例会在惰性底物上形成一条色带。结果显示该检测的灵敏度约为94.87%，特异性为85.71%，与酶联免疫吸附测定（ELISA）技术相当。该检测的高灵敏度和特异性为诊断这种寄生虫病提供了快速诊断的巨大优势。无需其他设备或程序。
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