生物素-抗生物素蛋白系统的应用、循环免疫复合物的测定以及不同包虫病患者抗体反应的评估。
Application of biotin-avidin system, determination of circulating immune complexes, and evaluation of antibody response in different hydatidosis patients.
作者信息
Shen Z Q, Feng X H, Qian Z X, Liu R L, Yang C R
机构信息
Department of Thoracocardiac Surgery, First Affiliated Hospital, Xinjiang Medical College, People's Republic of China.
出版信息
Am J Trop Med Hyg. 1988 Jul;39(1):93-6.
The avidin-biotin-peroxidase complex enzyme-linked immunosorbent assay (ABC-ELISA) and standard ELISA were used for the detection of Echinococcus granulosus antibody in sera of 101 patients operated on for hydatid disease, 40 patients with miscellaneous nonhydatid diseases, and 61 normal subjects. Sensitivity and specificity of the two procedures were comparable and the geometric mean antibody titer detected with ABC-ELISA was higher than with standard ELISA. The ABC-ELISA is a sensitive, specific, simple, and convenient method for diagnosing hydatidosis.
采用抗生物素蛋白-生物素-过氧化物酶复合物酶联免疫吸附测定法（ABC-ELISA）和标准ELISA检测101例因包虫病接受手术治疗患者、40例患有其他非包虫病的患者以及61名正常受试者血清中的细粒棘球绦虫抗体。两种检测方法的敏感性和特异性相当，ABC-ELISA检测到的几何平均抗体滴度高于标准ELISA。ABC-ELISA是一种诊断包虫病的灵敏、特异、简便且便捷的方法。
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