Conde P, Loureiro J, Santos C
Department of Biology, Campus Universitário de Santiago, University of Aveiro, 3810-193, Aveiro, Portugal.
Plant Cell Rep. 2004 Apr;22(9):632-9. doi: 10.1007/s00299-003-0735-1. Epub 2003 Nov 13.
Somatic embryogenesis from mature elm ( Ulmus minor Mill.) in vitro-cloned material is possible. Embryogenic callus was obtained from leaves inoculated on two different MS-based media-one supplemented with 2.3 microM 2,4-dichlorophenoxyacetic acid (I2) and the other supplemented with 1.1 microM kinetin (I6). However, only leaves cultured on medium I6 produced somatic embryos, at the globular stage, when embryogenic callus was maintained in induction media. When embryogenic callus from medium I6 was transferred to basal medium, somatic embryos with green cotyledons were obtained. An average of 35.9% of these embryos converted easily into normal plants in conversion medium with 1% sucrose. Acclimatisation reached 39.7%, and this was not significantly different from a control group consisting of plants propagated by axillary buds. No morphological differences were observed between plants derived from somatic embryos and control plants. Also, no differences in ploidy were detected between the somatic embryo-derived plants and the mother plants.
利用成熟榆树（小叶榆）的体外克隆材料进行体细胞胚胎发生是可行的。从接种在两种不同的基于MS的培养基上的叶片中获得了胚性愈伤组织，一种培养基添加了2.3微摩尔的2,4-二氯苯氧乙酸（I2），另一种添加了1.1微摩尔的激动素（I6）。然而，只有在培养基I6上培养的叶片在胚性愈伤组织保持在诱导培养基中时，在球形期产生了体细胞胚。当将来自培养基I6的胚性愈伤组织转移到基础培养基上时，获得了具有绿色子叶的体细胞胚。在含有1%蔗糖的转化培养基中，平均35.9%的这些胚很容易转化为正常植株。驯化率达到39.7%，这与由腋芽繁殖的植株组成的对照组没有显著差异。在源自体细胞胚的植株和对照植株之间未观察到形态差异。此外，在源自体细胞胚的植株和母本植株之间未检测到倍性差异。
