Leal F, Loureiro J, Rodriguez E, Pais M S, Santos C, Pinto-Carnide O
Center of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.
Plant Cell Rep. 2006 Sep;25(9):978-85. doi: 10.1007/s00299-006-0162-1. Epub 2006 Apr 14.
Flow cytometry was employed to determine the ploidy level of Vitis vinifera L. somatic embryo-derived plants obtained from anther culture. Only one among the 41 analysed plants (2.4%) presented somaclonal variation (tetraploidy); the other plants were diploid. No significant differences (P<or=0.05) were detected between diploid and parental field plants. No haploid or aneuploid plants were observed. The nuclear DNA content of nine V. vinifera cultivars was also estimated using flow cytometry. A non-significant variation was found among the cultivars, with DNA content ranging from 1.17 pg/2C (cv. 'Tinta Barroca' and 'Viosinho') to 1.26 pg/2C (cv. 'Cabernet Sauvignon'). These results and previous studies on other Vitis species suggest that Vitis genome is stable with regard to nuclear DNA content.
采用流式细胞术测定从花药培养获得的葡萄（Vitis vinifera L.）体细胞胚再生植株的倍性水平。在41株被分析的植株中，只有1株（2.4%）出现体细胞克隆变异（四倍体）；其他植株均为二倍体。二倍体植株与亲本田间植株之间未检测到显著差异（P≤0.05）。未观察到单倍体或非整倍体植株。还使用流式细胞术估计了9个葡萄品种的核DNA含量。各品种之间发现无显著差异，DNA含量范围为1.17 pg/2C（‘添普兰尼洛’和‘维奥西尼奥’品种）至1.26 pg/2C（‘赤霞珠’品种）。这些结果以及之前对其他葡萄品种的研究表明，就核DNA含量而言，葡萄基因组是稳定的。
