Valentine Stephen J, Koeniger Stormy L, Clemmer David E
Beyond Genomics, 40 Bear Hill Road, Waltham, Massachusetts 02451, USA.
Anal Chem. 2003 Nov 15;75(22):6202-8. doi: 10.1021/ac030111r.
A new ion mobility instrument that incorporates a low-field region for ion separation and a high-field region for collisional activation is described. In this approach, mixtures of ions are separated based on differences in their mobilities in a approximately 20-cm-long low-field ( approximately 5 V cm(-)(1)) region of a drift tube. As the ions approach the drift tube exit, they are exposed to a large focusing potential drop; at high fields, ions are efficiently collisionally activated and dissociate as they exit the drift tube. We have demonstrated this approach by examining the fragmentation pattern for electrosprayed bradykinin ions. These studies show that the activation process is highly tunable; it is possible to modulate the field such that precursor ion mass spectra as well as several high-field collision-induced fragmentation patterns can be obtained. The approach also appears to be a simple means of activating protein ions, as demonstrated by examining electrosprayed myoglobin ions.
本文描述了一种新型离子迁移谱仪，该仪器包含用于离子分离的低场区域和用于碰撞活化的高场区域。在这种方法中，离子混合物基于其在漂移管中约20厘米长的低场（约5 V cm⁻¹）区域内迁移率的差异进行分离。当离子接近漂移管出口时，它们会受到一个大的聚焦电势降；在高场下，离子在离开漂移管时会被有效地碰撞活化并解离。我们通过研究电喷雾缓激肽离子的碎裂模式证明了这种方法。这些研究表明，活化过程具有高度可调性；可以调节电场，从而获得前体离子质谱以及几种高场碰撞诱导的碎裂模式。通过研究电喷雾肌红蛋白离子，该方法似乎也是活化蛋白质离子的一种简单手段。
