El'nikova S G, Pankova T M, Starostina M V, Shtark M B
Ontogenez. 1992 Sep-Oct;23(5):480-6.
In order to study the molecular mechanisms of neurogenesis, monoclonal antibodies (MAbs) were produced against antigens of the developing rat hippocampus. MAb 3G7-F8 was used for immunohistochemical localization of the corresponding antigen of paraffin sections of the rat brain at days 0, 5, 14, and 21 of the postnatal development. In the hippocampus of newborn and 5-day-old rats, positive immunostaining was observed in the cytoplasm and proximal segments of processes of neurons located in granular, polymorph, and pyramidal layers, as well as in entorhinal cortex. In granule cell bodies and neurons of entorhinal cortex specific staining decreased by day 14 and disappeared by day 21 after birth, whereas neurons of pyramidal and polymorph layers remained immunopositive. Diffuse specific staining in the cerebellum was observed beginning from day 5 after birth in the Purkinje cell layer. On days 14-21 positive reaction was observed in Purkinje cell bodies and in the layer containing dendrites of Purkinje cells and parallel fibers. External and internal granular layers remained immunonegative. No specific staining was observed in other regions of the brain, as well as in the control slices. These data suggest that the antigen detected by the 3G7-F8 antibody is involved in the formation of the neuronal connections.
为了研究神经发生的分子机制，制备了针对发育中大鼠海马体抗原的单克隆抗体（MAb）。MAb 3G7-F8用于对出生后发育第0、5、14和21天大鼠脑石蜡切片的相应抗原进行免疫组织化学定位。在新生和5日龄大鼠的海马体中，在颗粒层、多形层和锥体细胞层以及内嗅皮质的神经元胞质和突起近端观察到阳性免疫染色。出生后第14天，颗粒细胞体和内嗅皮质神经元的特异性染色减少，第21天消失，而锥体细胞层和多形层的神经元仍呈免疫阳性。出生后第5天开始在小脑浦肯野细胞层观察到弥漫性特异性染色。在第14 - 21天，在浦肯野细胞体以及含有浦肯野细胞树突和平行纤维的层中观察到阳性反应。外颗粒层和内颗粒层仍呈免疫阴性。在脑的其他区域以及对照切片中未观察到特异性染色。这些数据表明，3G7-F8抗体检测到的抗原参与了神经元连接的形成。
