Lagenaur C, Schachner M
J Supramol Struct Cell Biochem. 1981;15(4):335-46. doi: 10.1002/jsscb.1981.380150404.
A monoclonal antibody designated M2 arose from the fusion of mouse myeloma cells with splenocytes from a rat immunized with particulate fraction from early postnatal mouse cerebellum. Expression of M2 antigen was examined by indirect immunofluorescence on frozen sections of developing and adult mouse cerebellum and on monolayer cultures of early postnatal mouse cerebellar cells. In adult cerebellum, M2 staining outlines the cell bodies of granule and Purkinje cells. A weaker, more diffuse staining is seen in the molecular layer and white matter. In sections of newborn cerebellum, M2 antigen is weakly detectable surrounding cells of the external granular layer and Purkinje cells. The expression of M2 antigen increases during development in both cell types, reaching adult levels by postnatal day 14. At all stages of postnatal cerebellar development, granule cells that have completed migration to the internal granule layer are more heavily stained by M2 antibodies than are those before and in process of migration. In monolayer cultures, M2 antigen is detected on the cell surface of all GFA protein-positive astrocytes and on more immature oligodendrocytes that express 04 antigen but not 01 antigen. After 3 days in culture, tetanus toxin positive neurons begin to express M2 antigen. The same delayed expression of M2 antigen on neurons is observed in cultures derived from mice ranging in age from postnatal day 0 to 10.
一种名为M2的单克隆抗体源自小鼠骨髓瘤细胞与用出生后早期小鼠小脑颗粒部分免疫的大鼠脾细胞的融合。通过间接免疫荧光法在发育中和成年小鼠小脑的冰冻切片以及出生后早期小鼠小脑细胞的单层培养物上检测M2抗原的表达。在成年小脑中，M2染色勾勒出颗粒细胞和浦肯野细胞的细胞体。在分子层和白质中可见较弱、更弥散的染色。在新生小脑切片中，在外部颗粒层细胞和浦肯野细胞周围可微弱检测到M2抗原。在这两种细胞类型中，M2抗原的表达在发育过程中增加，在出生后第14天达到成年水平。在出生后小脑发育的所有阶段，已迁移至内颗粒层的颗粒细胞比迁移前和正在迁移的颗粒细胞被M2抗体染色更浓。在单层培养物中，在所有GFA蛋白阳性星形胶质细胞以及表达04抗原但不表达01抗原的更不成熟的少突胶质细胞的细胞表面检测到M2抗原。培养3天后，破伤风毒素阳性神经元开始表达M2抗原。在出生后0至10天不同年龄小鼠来源的培养物中观察到神经元上M2抗原的相同延迟表达。
