Mahé E, Bodemer C, Descamps V, Mahé I, Crickx B, De Prost Y, Favre M
Unité des Papillomavirus, Institut Pasteur, Paris, France.
Br J Dermatol. 2003 Oct;149(4):819-25. doi: 10.1046/j.1365-2133.2003.05587.x.
Psoriasis is a T-cell-mediated immunological disease characterized by epidermal proliferation. The nature of the antigen(s) responsible for T-cell activation is still unknown. It has been suggested that the human papillomaviruses (HPVs) associated with epidermodysplasia verruciformis (EV), including the oncogenic HPV5, may contribute to the pathogenesis of psoriasis.
To determine whether EV-HPVs may play a role early in the disease, we searched for these viruses in children with psoriasis. The influence of clinical data on EV-HPV infection was investigated.
We studied scrapings of involved skin from 26 children aged 1.5-13 years with psoriasis. As controls, we analysed scrapings from 28 adults with psoriasis and 15 children with atopic dermatitis, as well as scrapings from normal skin of 28 adults with no known history of HPV infection. We searched for EV-HPV DNA sequences with a nested polymerase chain reaction method using degenerate primers specific for EV-HPVs and primers specific for HPV5 and HPV36, two EV-HPVs frequently detected in adults with psoriasis.
Similar high prevalences were observed in children and adults with psoriasis for EV-HPVs (38.5% vs. 35.7%), HPV5 (46.2% vs. 46.4%) and HPV36 (15.4% vs. 25.0%). As in adults, we found several EV-HPV genotypes and HPV5 and HPV36 variants. A novel HPV36 subtype, HPV36b, was identified. Lower prevalences were observed in children with atopic dermatitis and in adults from the general population (6.7-10.1%). No correlation was observed between frequency of detection of HPVs and clinical data. It is noteworthy that HPV5 was identified in an 18-month-old girl and in a boy with psoriasis developing for only 1 week.
The early detection of several EV-HPV genotypes in children further supports the link between psoriasis and EV-HPVs and suggests a putative role for these viruses in the pathogenesis of psoriasis.
银屑病是一种以表皮增殖为特征的T细胞介导的免疫性疾病。导致T细胞活化的抗原性质仍不清楚。有人提出，与疣状表皮发育不良（EV）相关的人乳头瘤病毒（HPV），包括致癌性HPV5，可能参与银屑病的发病机制。
为了确定EV-HPV是否可能在疾病早期发挥作用，我们在银屑病患儿中寻找这些病毒。研究了临床数据对EV-HPV感染的影响。
我们研究了26名年龄在1.5至13岁之间的银屑病患儿受累皮肤的刮屑。作为对照，我们分析了28名成年银屑病患者、15名特应性皮炎患儿的刮屑，以及28名无HPV感染病史的正常成年人正常皮肤的刮屑。我们使用针对EV-HPV的简并引物以及针对HPV5和HPV36（在成年银屑病患者中经常检测到的两种EV-HPV）的引物，通过巢式聚合酶链反应方法寻找EV-HPV DNA序列。
银屑病患儿和成年患者中EV-HPV（38.5%对35.7%）、HPV5（46.2%对46.4%）和HPV36（15.4%对25.0%）的患病率相似。与成年人一样，我们发现了几种EV-HPV基因型以及HPV5和HPV36变体。鉴定出一种新的HPV36亚型HPV36b。特应性皮炎患儿和普通人群中的成年人患病率较低（6.7 - 10.1%）。未观察到HPV检测频率与临床数据之间的相关性。值得注意的是，在一名18个月大的女孩和一名病程仅1周的银屑病男孩中发现了HPV5。
在儿童中早期检测到几种EV-HPV基因型进一步支持了银屑病与EV-HPV之间的联系，并表明这些病毒在银屑病发病机制中可能具有假定作用。
