自体移植脾组织的吞噬能力下降。
Decreased phagocytic capacity of autotransplanted splenic tissue.
作者信息
Smith Eric, J De Young Neville, Drew Paul A
机构信息
Department of Surgery, The University of Adelaide, South Australia, Australia.
出版信息
ANZ J Surg. 2003 Nov;73(11):894-6. doi: 10.1046/j.1445-2197.2003.02819.x.
BACKGROUND
Asplenic patients have an increased risk of infections. Operations such as autotransplantation have been proposed to restore functional splenic tissue after splenectomy, but the protective value of this tissue is unclear. Immune responses such as production of antibody remain impaired in humans and animals even when such tissue is present, and clearance of particles from the blood is reported to be less efficient than by normal spleen tissue. The present study investigated the phagocytic capacity of cells in the regenerated tissue in vitro, free of the confounding effects of hepatic clearance.
METHODS
Single cell suspensions were prepared from splenic tissue from rats 6 months after splenic autotransplantation or sham operation. Phagocytosis of killed, fluorescein-labelled bacteria was measured by flow cytometry.
RESULTS
Autotransplanted tissue contained fewer phagocytic cells than normal tissue, and these cells phagocytosed less per cell. Phagocytosis by spleen cells was dependent on heat-labile opsonic factors.
CONCLUSIONS
Autotransplanted splenic tissue does not restore the phagocytic capacity lost following splenectomy.
背景
无脾患者感染风险增加。有人提出进行自体移植等手术，以在脾切除术后恢复功能性脾组织，但这种组织的保护价值尚不清楚。即使存在这种组织，人类和动物的抗体产生等免疫反应仍然受损，并且据报道，与正常脾组织相比，从血液中清除颗粒的效率较低。本研究在体外研究了再生组织中细胞的吞噬能力，排除了肝脏清除的混杂影响。
方法
从自体脾移植或假手术6个月后的大鼠脾组织制备单细胞悬液。通过流式细胞术测量对经荧光素标记的死细菌的吞噬作用。
结果
自体移植组织中的吞噬细胞比正常组织少，并且这些细胞每个细胞的吞噬量也较少。脾细胞的吞噬作用依赖于热不稳定的调理素因子。
结论
自体移植的脾组织不能恢复脾切除术后丧失的吞噬能力。
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