Kroin Jeffrey S, Buvanendran Asokumar, Nagalla Subhash K S, Tuman Kenneth J
Department of Anesthesiology, Rush Medical College, Chicago, Illinois 60612, USA.
Can J Anaesth. 2003 Nov;50(9):904-8. doi: 10.1007/BF03018737.
Controversy exists concerning the influence of gender on pain sensitivity and response to analgesics both in animal and human studies. The present study compares postoperative pain scores in male and female rats and how they respond to analgesic interventions.
Unilateral plantar foot incisions were made in Sprague-Dawley rats of both genders, producing mechanical allodynia in an established model of postoperative pain. Postoperative pain scores were monitored for four days following incision to identify intrinsic differences between the two groups. Animals were tested with analgesics (opioids, alpha(2)-adrenergic agonists, acetylcholine esterase inhibitors, gabapentin) both systemically and intrathecally on the day after incision to assess gender differences in the anti-allodynic effect of these drugs.
In the plantar foot incision model of postoperative pain there was no gender difference in postoperative mechanical hypersensitivity (von Frey filaments) over four days. Morphine (3 mg x kg(-1)) and gabapentin (25 mg x kg(-1)) administered intraperitoneally decreased postoperative mechanical hypersensitivity, but with no gender difference. Intrathecal morphine (1-2 nmol), gabapentin (60-120 nmol), clonidine (45 nmol), and neostigmine (6.6 nmol) also showed no gender difference in analgesic effect.
The results of this study demonstrate that in Sprague-Dawley rats there are no gender differences in postoperative pain perception or the response to analgesics, indicating that this strain of rats can be used without introducing gender bias in studies of postoperative pain.
在动物和人体研究中，性别对疼痛敏感性及镇痛药反应的影响存在争议。本研究比较了雄性和雌性大鼠的术后疼痛评分以及它们对镇痛干预的反应。
在雌雄Sprague-Dawley大鼠的单侧足底做切口，在已建立的术后疼痛模型中产生机械性异常性疼痛。切口后监测四天的术后疼痛评分，以确定两组之间的内在差异。在切口后第二天，对动物进行全身和鞘内注射镇痛药（阿片类药物、α₂肾上腺素能激动剂、乙酰胆碱酯酶抑制剂、加巴喷丁）测试，以评估这些药物抗异常性疼痛作用的性别差异。
在足底切口术后疼痛模型中，四天内术后机械性超敏反应（von Frey细丝）无性别差异。腹腔注射吗啡（3mg·kg⁻¹）和加巴喷丁（25mg·kg⁻¹）可降低术后机械性超敏反应，但无性别差异。鞘内注射吗啡（1 - 2nmol）、加巴喷丁（60 - 120nmol）、可乐定（45nmol）和新斯的明（6.6nmol）在镇痛效果上也无性别差异。
本研究结果表明，在Sprague-Dawley大鼠中，术后疼痛感知或对镇痛药的反应无性别差异，这表明在术后疼痛研究中使用该品系大鼠不会引入性别偏差。
