[血浆极低密度脂蛋白、低密度脂蛋白及高密度脂蛋白对内源性高甘油三酯血症患者血小板聚集的影响]

[Effects of plasma very low density lipoprotein, low density lipoprotein and high density lipoprotein on platelet aggregation in endogenous hypertriglyceridemia].

作者信息

Deng Zuyue, Liu Bingwen, Zhou Jing, Zhang Zuhui, Liu Yu, Bai Huai

机构信息

Apolipoprotern Research Unit, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Oct;34(4):704-7.

PMID:14619587

Abstract

OBJECTIVE

To study whether plasma very low density lipoprotein (VLDL), low density lipoprotein (LDL), high density lipoprotein (HDL) were oxidatively modified in endogenous hypertriglyceridemia (HTG) and to investigate the effects of HTG VLDL, LDL and HDL on platelet aggregation in vitro.

METHODS

Blood samples were taken from 21 patients with endogenous triglyceridemia and 21 normal healthy subjects; these two groups were similar in respect to age and sex. Their plasma VLDL, LDL and HDL were isolated by density gradient ultracentrifugation method, and plasma triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDLC) were measured by enzyme method. The oxidative modification of LDL, VLDL and HDL was identified by agarose gel relative electrophoretic mobility (REM), absorbance at 234 nm (A234) and fluorescence of thiobarbituric acid reaction substances (TBARS). With the reaction system that consisted of mixed fresh normal plasma, platelet aggregation was induced by adenosine diphosphate (ADP), and the platelet maximal aggregation rate (MAR) was recorded on a 4-channel light aggregometer.

RESULTS

The plasma TG, TBARS levels in HTG group were 1.6 and 0.4 times over those of the control group respectively (P < 0.01). The plasma HDLC in HTG group was 32% lower than those of the control group (P < 0.01). REM, A234 and TBARS of VLDL, LDL and HDL in HTG group were significantly higher than those in the control group (P < 0.01). MAR of VLDL, LDL and HDL in HTG group were significantly higher than those in the control group (P < 0.05). The correlation analysis indicated that REM, A234 and TBARS of LDL and HDL in HTG group were positively correlated with MAR (P < 0.01).

CONCLUSION

The above data indicated that oxidative modification of plasma VLDL, LDL, HDL did occur in endogenous hypertriglyceridemia in vivo, and VLDL, LDL and HDL enhanced platelet aggregation in vitro.

摘要

目的

研究内源性高甘油三酯血症（HTG）患者血浆极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）是否发生氧化修饰，并探讨HTG患者的VLDL、LDL和HDL对体外血小板聚集的影响。

方法

采集21例内源性高甘油三酯血症患者和21例正常健康受试者的血样；两组在年龄和性别方面相似。采用密度梯度超速离心法分离其血浆VLDL、LDL和HDL，用酶法测定血浆甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDLC）。通过琼脂糖凝胶相对电泳迁移率（REM）、234nm处吸光度（A234）和硫代巴比妥酸反应物质（TBARS）荧光鉴定LDL、VLDL和HDL的氧化修饰。在由新鲜正常混合血浆组成的反应体系中，用二磷酸腺苷（ADP）诱导血小板聚集，在四通道光聚集仪上记录血小板最大聚集率（MAR）。

结果

HTG组血浆TG、TBARS水平分别是对照组的1.6倍和0.4倍（P<0.01）。HTG组血浆HDLC比对照组低32%（P<0.01）。HTG组VLDL、LDL和HDL的REM、A234和TBARS显著高于对照组（P<0.01）。HTG组VLDL、LDL和HDL的MAR显著高于对照组（P<0.05）。相关性分析表明，HTG组LDL和HDL的REM、A234和TBARS与MAR呈正相关（P<0.01）。