Kondou I, Nakada J, Hishinuma H, Masuda F, Machida T, Endou H
Department of Urology, Tokyo Jikeikai University, Japan.
Ren Fail. 1992;14(4):479-83. doi: 10.3109/08860229209047656.
This study was designed to determine changes in one metabolic function, gluconeogenesis (GLG), after ischemic renal injury. Tubule suspensions were prepared by collagenase treatment of SD rat kidneys on 1, 3, and 7 days after left renal artery and vein occlusion for 0-90 min and incubated in Krebs-Henseleit buffer with or without 2 mM pyruvate or malate aerobically. Glucose contents were assayed photometrically. On days 1 and 3 after ischemia for longer than 60 min, serum creatinine levels rose significantly. The tendency of increase of GLG was observed on days 1 and 3 after 10-60 min of ischemia. GLG increased significantly on day 1 after 30-min ischemia. On the other hand, GLG decreased significantly on day 1 after 90-min treatment. Morphologic damage was limited to the corticomedullary region on days 1 and 3 after ischemic times of 30 and 60 min. These results suggest that renal GLG is stimulated to supply energy for ATP decrease by ischemia and for further regeneration in extraproximal segments along the nephron.
本研究旨在确定缺血性肾损伤后一种代谢功能即糖异生（GLG）的变化。在左肾动脉和静脉闭塞0 - 90分钟后的第1、3和7天，通过胶原酶处理SD大鼠肾脏制备肾小管悬液，并在含有或不含有2 mM丙酮酸或苹果酸的Krebs - Henseleit缓冲液中进行需氧孵育。通过光度法测定葡萄糖含量。缺血超过60分钟后的第1天和第3天，血清肌酐水平显著升高。在缺血10 - 60分钟后的第1天和第3天观察到GLG增加的趋势。缺血30分钟后第1天GLG显著增加。另一方面，90分钟处理后第1天GLG显著降低。缺血30分钟和60分钟后的第1天和第3天，形态学损伤仅限于皮质髓质区域。这些结果表明，肾脏GLG受到刺激，为缺血导致的ATP减少以及肾单位近端以外节段的进一步再生提供能量。
