Aliya S, Reddanna P, Thyagaraju K
Department of Biochemistry, S. V. University, Tirupathi, India.
Mol Cell Biochem. 2003 Nov;253(1-2):319-27. doi: 10.1023/a:1026036521852.
Glutathione S-transferases (GSTs, EC 2.5.1.18) are multifunctional and multigene products. They are versatile enzymes and participate in the nucleophilic attack of the sulphur atom of glutathione on the electrophilic centers of various endogenous and xenobiotic compounds. Out of the five, alpha, micro, pi, sigma and theta, major classes of GSTs, GST-pi has significance in the diagnosis of cancers as it is expressed abundantly in tumor cells. This protein is a single gene product, coded by seven exons, that is having 24 kDa mass and pI value of 7.0. Four upstream elements such as two enhancers, and one of each of AP-1 site and GC box regulate pi gene. During chemical carcinogenesis because of jun/fos oncogenes (AP-1) regulatory elements, specifically GST-pi is expressed in liver. Therefore this gene product could be used as marker protein for the detection of chemical toxicity and carcinogenesis.
谷胱甘肽S-转移酶（GSTs，EC 2.5.1.18）是多功能且由多个基因编码的产物。它们是多功能酶，参与谷胱甘肽硫原子对各种内源性和外源性化合物亲电中心的亲核攻击。在GSTs的五个主要类别（α、μ、π、σ和θ）中，GST-π在癌症诊断中具有重要意义，因为它在肿瘤细胞中大量表达。这种蛋白质是由单个基因编码的产物，由七个外显子编码，分子量为24 kDa，pI值为7.0。四个上游元件，如两个增强子、一个AP-1位点和一个GC盒，调节π基因。在化学致癌过程中，由于jun/fos癌基因（AP-1）调控元件，GST-π特别是在肝脏中表达。因此，这种基因产物可作为检测化学毒性和致癌作用的标记蛋白。
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