Ruehl-Fehlert Christine, Kittel Birgit, Morawietz Gerd, Deslex Paul, Keenan Charlotte, Mahrt Charles R, Nolte Thomas, Robinson Mervyn, Stuart Barry P, Deschl Ulrich
Department of Toxicologic Pathology, Bayer AG, Wuppertal, Germany.
Exp Toxicol Pathol. 2003 Sep;55(2-3):91-106.
This is the first part of a series of three articles on trimming instructions of rat and mouse protocol organs and tissues in regulatory type toxicity studies. It is based on the experience made in the European RITA and American NACAD working groups and is an extended revision of trimming guides published in 1995 (Bahnemann et al.). The optimum localization for tissue preparation, the sample size, the direction of sectioning and the number of sections to be prepared is described organ by organ. These descriptions are illustrated for each organ by a schematic drawing and a macro-photograph showing the plane of section as well as a low power view of the H&E stained slide demonstrating the optimum "end-product". This revision will improve the quality and efficiency of routine procedures and facilitate daily work in the histotechnical lab. It will promote intra- and inter-study reproducibility and comparability and thus lead to a further coherence within each study and improvement of the validity of historical control data.
这是关于监管类型毒性研究中大鼠和小鼠实验动物器官及组织修整指南系列三篇文章的第一篇。它基于欧洲RITA和美国NACAD工作组的经验，是1995年发表的修整指南（Bahnemann等人）的扩展修订版。本文逐器官描述了组织制备的最佳定位、样本大小、切片方向以及要制备的切片数量。每个器官的这些描述都配有示意图和宏观照片进行说明，示意图和宏观照片展示了切片平面以及苏木精-伊红（H&E）染色玻片的低倍视野，呈现出最佳的“最终产物”。此次修订将提高常规程序的质量和效率，便于组织技术实验室的日常工作。它将促进研究内部和研究之间的可重复性和可比性，从而在每项研究中实现更高的连贯性，并提高历史对照数据的有效性。
