Iemitsu Motoyuki, Miyauchi Takashi, Maeda Seiji, Sakai Satoshi, Fujii Nobuharu, Miyazaki Hitoshi, Kakinuma Yoshihiko, Matsuda Mitsuo, Yamaguchi Iwao
Cardiovascular Division, Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Japan.
Hypertens Res. 2003 Oct;26(10):829-37. doi: 10.1291/hypres.26.829.
Hypertension-induced pathological cardiac hypertrophy (hypertensive heart) and exercise training-induced physiological cardiac hypertrophy (athletic heart) have differences in cardiac properties. We hypothesized that gene expression of energy metabolic enzymes differs between these two types of cardiac hypertrophy. To investigate whether mRNA expression of key enzymes in the long-chain fatty acid (FA), glucose, and lactic acid metabolic pathways differs between these two types of cardiac hypertrophy, we used the hearts of spontaneously hypertensive rats (SHR; 19 weeks old) as a model of the hypertensive heart, swim-trained rats (Trained; 19 weeks old, swimming training for 15 weeks) as a model of the athletic heart, and sedentary Wistar-Kyoto rats (Control; 19 weeks old). SHR developed hypertensive cardiac hypertrophy, of which cardiac function was deteriorated, whereas Trained rats developed an athletic heart, of which cardiac function was enhanced. The mRNA expression of CD36, which involved in uptake of long-chain FA, in the heart was almost never detected in the SHR group. Furthermore, the mRNA expression of key enzymes in the long-chain FA metabolic pathway (acyl CoA synthase [ACoAS], carnitine palmitoyl transferase [CPT]-I, CPT-II, and isocitrate dehydrogenase [ISCD]) in the heart was significantly higher in the SHR group compared with the Control group. The mRNA expression of ACoAS, CPT-I, ISCD, and CD36 in the heart did not differ between Trained group and Control group, whereas that of CPT-II in the Trained group was significantly higher compared with the Control group. The mRNA expression of key enzymes (phosphofructokinase and lactate dehydrogenase) in glycolytic metabolic pathway in the heart was markedly higher in the SHR group compared with the Control group, whereas these mRNA expressions did not differ between Trained group and Control group. These findings suggest that the molecular phenotypes in the energy metabolic system differ in hypertension-induced pathological and exercise training-induced physiological cardiac hypertrophy, and these differences may participate in the differences in cardiac function.
高血压诱导的病理性心脏肥大（高血压性心脏）和运动训练诱导的生理性心脏肥大（运动员心脏）在心脏特性方面存在差异。我们推测，这两种类型的心脏肥大中能量代谢酶的基因表达有所不同。为了研究长链脂肪酸（FA）、葡萄糖和乳酸代谢途径中关键酶的mRNA表达在这两种类型的心脏肥大之间是否存在差异，我们使用自发性高血压大鼠（SHR，19周龄）的心脏作为高血压性心脏的模型，经游泳训练的大鼠（训练组，19周龄，进行15周游泳训练）的心脏作为运动员心脏的模型，以及久坐不动的Wistar-Kyoto大鼠（对照组，19周龄）。SHR出现了高血压性心脏肥大，其心脏功能恶化，而训练组大鼠出现了运动员心脏，其心脏功能增强。在SHR组中，几乎未检测到心脏中参与长链FA摄取的CD36的mRNA表达。此外，与对照组相比，SHR组心脏中长链FA代谢途径中关键酶（酰基辅酶A合成酶[ACoAS]、肉碱棕榈酰转移酶[CPT]-I、CPT-II和异柠檬酸脱氢酶[ISCD]）的mRNA表达显著更高。训练组和对照组心脏中ACoAS、CPT-I、ISCD和CD36的mRNA表达没有差异，而训练组中CPT-II的mRNA表达与对照组相比显著更高。与对照组相比，SHR组心脏中糖酵解代谢途径中关键酶（磷酸果糖激酶和乳酸脱氢酶）的mRNA表达明显更高，而训练组和对照组之间这些mRNA表达没有差异。这些发现表明，能量代谢系统中的分子表型在高血压诱导的病理性和运动训练诱导的生理性心脏肥大中有所不同，这些差异可能参与了心脏功能的差异。
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