Wang Tutu, Ning Miaomiao, Mo Yurou, Tian Xinyu, Fu Yu, Laher Ismail, Li Shunchang
Institute of Sports Medicine and Health, Chengdu Sport University, Chengdu 610041, China.
Department of Anesthesiology, Pharmacology and Therapeutics, Faculty of Medicine, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Antioxidants (Basel). 2024 Sep 26;13(10):1167. doi: 10.3390/antiox13101167.
The increasing prevalence of type 2 diabetes mellitus (T2DM) leads to significant global health challenges, including cardiac structural and functional deficits, which in severe cases can progress to heart failure that can further strain healthcare resources. Aerobic exercise can ameliorate cardiac dysfunction in individuals with diabetes, although a comprehensive understanding of its underlying mechanisms remains elusive. This study utilizes untargeted metabolomics to reveal aerobic-exercise-activated metabolic biomarkers in the cardiac tissues of Sprague Dawley rats with T2DM. Metabolomics analysis revealed that diabetes altered 1029 myocardial metabolites, while aerobic exercise reversed 208 of these metabolites, of which 112 were upregulated and 96 downregulated. Pathway topology analysis suggested that these metabolites predominantly contributed to purine metabolism and arginine biosynthesis. Furthermore, receiver operating characteristic curve analysis identified 10 potential biomarkers, including xanthine, hypoxanthine, inosine, dGMP, l-glutamic acid, l-arginine, l-tryptophan, (R)-3-hydroxybutyric acid, riboflavin, and glucolepidiin. Finally, data from Pearson correlation analysis indicated that some metabolic biomarkers strongly correlated with cardiac function. Our data suggest that certain metabolic biomarkers play an important role in ameliorating diabetes-related cardiac dysfunction by aerobic exercise.
2型糖尿病（T2DM）患病率的不断上升给全球健康带来了重大挑战，包括心脏结构和功能缺陷，在严重情况下可发展为心力衰竭，这会进一步加重医疗资源的负担。有氧运动可以改善糖尿病患者的心脏功能障碍，尽管对其潜在机制的全面了解仍然难以捉摸。本研究利用非靶向代谢组学来揭示T2DM的Sprague Dawley大鼠心脏组织中有氧运动激活的代谢生物标志物。代谢组学分析显示，糖尿病改变了1029种心肌代谢物，而有氧运动逆转了其中208种代谢物，其中112种上调，96种下调。通路拓扑分析表明，这些代谢物主要参与嘌呤代谢和精氨酸生物合成。此外，受试者工作特征曲线分析确定了10种潜在生物标志物，包括黄嘌呤、次黄嘌呤、肌苷、dGMP、L-谷氨酸、L-精氨酸、L-色氨酸、（R）-3-羟基丁酸、核黄素和葡糖列平。最后，Pearson相关分析数据表明，一些代谢生物标志物与心脏功能密切相关。我们的数据表明，某些代谢生物标志物在有氧运动改善糖尿病相关心脏功能障碍中起重要作用。
