Gemmell E, Winning T A, Carter C L, Ford P J, Bird P S, Ashman R B, Grieco D A, Seymour G J
Immunopathology Laboratory, Oral Biology and Pathology, School of Dentistry, The University of Queensland, Brisbane, Australia.
Oral Microbiol Immunol. 2003 Dec;18(6):364-70. doi: 10.1046/j.0902-0055.2002.00098.x.
This study examined the nature of the infiltrating cells in Porphyromonas gingivalis-induced lesions and immunoglobulins in the serum samples of BALB/c (H-2d), C57BL6 (H-2b), DBA/2J (H-2d) and CBA/CaH (H-2k) mice. Mice were immunized intraperitoneally with P. gingivalis outer membrane antigens or sham-immunized with phosphate-buffered saline followed by subcutaneous challenge with live organisms 1 week after the final immunization. The resulting skin abscesses were excised 7 days later, cryostat sections cut and an immunoperoxidase method used to detect the presence of CD4+ and CD8+ T-cell subsets, CD14+ macrophages and CD19+ B cells. Peroxidase positive neutrophils and IgG1- and IgG2a-producing plasma cells were also identified. Anti P. gingivalis IgG1 and IgG2a subclass antibodies were determined in serum obtained by cardiac puncture. Very few CD8+ T cells and CD19+ B cells were found in any of the lesions. The percentages of CD4+ cells, CD14+ cells and neutrophils were similar in lesions of immunized BALB/c and C57BL6 mice, with a trend towards a higher percentage of CD14+ cells in sham-immunized mice. The percentage of CD14+ cells was higher than that of CD4+ cells in immunized compared with sham-immunized DBA/2J mice. The percentages of CD4+ and CD14+ cells predominated in immunized CBA/CaH mice and CD4+ cells in sham-immunized CBA/CaH mice. The percentage of neutrophils in immunized CBA/CaH mice was significantly lower than that of CD14+ cells and CD4+ cells in sham-immunized mice. IgG1+ plasma cells were more dominant than IgG2a+ cells in immunized BALB/c, C57BL6 and DBA/2J mice, whereas IgG2a+ plasma cells were more obvious in sham-immunized mice. IgG2a+ plasma cells were predominant in immunized and sham-immunized CBA/CaH mice. In the serum, specific anti-P. gingivalis IgG2a antibody levels (Th1 response) were higher than IgG1 levels (Th2 response) in sham-immunized CBA/CaH and DBA/2J mice. In immunized BALB/c mice, IgG2a levels were lower than IgG1 levels, while IgG2a levels were higher in immunized C57BL6 mice. In conclusion, this study has shown differences in the proportion of infiltrating leukocytes and in the subclasses of immunoglobulin produced locally and systemically in response to P. gingivalis in different strains of mice, suggesting a degree of genetic control over the response to P. gingivalis.
本研究检测了牙龈卟啉单胞菌诱导的病变中浸润细胞的性质以及BALB/c（H-2d）、C57BL6（H-2b）、DBA/2J（H-2d）和CBA/CaH（H-2k）小鼠血清样本中的免疫球蛋白。小鼠腹腔注射牙龈卟啉单胞菌外膜抗原进行免疫，或用磷酸盐缓冲盐水进行假免疫，在末次免疫1周后皮下接种活菌。7天后切除形成的皮肤脓肿，制作冰冻切片，采用免疫过氧化物酶法检测CD4+和CD8+T细胞亚群、CD14+巨噬细胞和CD19+B细胞的存在情况。还鉴定了过氧化物酶阳性的中性粒细胞以及产生IgG1和IgG2a的浆细胞。通过心脏穿刺获取血清，检测抗牙龈卟啉单胞菌IgG1和IgG2a亚类抗体。在任何病变中均发现极少的CD8+T细胞和CD19+B细胞。免疫的BALB/c和C57BL6小鼠病变中CD4+细胞、CD14+细胞和中性粒细胞的百分比相似，假免疫小鼠中CD14+细胞百分比有升高趋势。与假免疫的DBA/2J小鼠相比，免疫的DBA/2J小鼠中CD14+细胞百分比高于CD4+细胞。免疫CBA/CaH小鼠中CD4+和CD14+细胞百分比占主导，假免疫CBA/CaH小鼠中CD4+细胞占主导。免疫CBA/CaH小鼠中中性粒细胞百分比显著低于假免疫小鼠中的CD14+细胞和CD4+细胞。在免疫的BALB/c、C57BL6和DBA/2J小鼠中，IgG1+浆细胞比IgG2a+细胞更占优势，而在假免疫小鼠中IgG2a+浆细胞更明显。在免疫和假免疫CBA/CaH小鼠中，IgG2a+浆细胞占主导。在血清中，假免疫的CBA/CaH和DBA/2J小鼠中特异性抗牙龈卟啉单胞菌IgG2a抗体水平（Th1反应）高于IgG1水平（Th2反应）。在免疫的BALB/c小鼠中，IgG2a水平低于IgG1水平，而在免疫的C57BL6小鼠中IgG2a水平较高。总之，本研究表明不同品系小鼠对牙龈卟啉单胞菌产生反应时，浸润白细胞比例以及局部和全身产生的免疫球蛋白亚类存在差异，提示对牙龈卟啉单胞菌的反应存在一定程度的基因控制。
