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The production of 6-deoxy-L-talan from Actinobacillus actinomycetemcomitans via bacterial coupling in vitro.

作者信息

Suzuki N, Nakano Y

机构信息

Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka, Japan.

出版信息

Oral Microbiol Immunol. 2003 Dec;18(6):401-4. doi: 10.1046/j.0902-0055.2003.00088.x.

DOI:10.1046/j.0902-0055.2003.00088.x
PMID:14622348
Abstract

A method of producing 6-deoxy-L-talan, the serotype c-specific polysaccharide antigen (SPA) from Actinobacillus actinomycetemcomitans,was established using a whole-cell reaction with two recombinant Escherichia coli strains. The production of serotype c-SPA was investigated using the dot blot assay with anti-A. actinomycetemcomitans NCTC 9710 serum after an 18-h reaction, starting with a solution containing the recombinant E. coli cells, alpha-d-glucose-1-phosphate, and dTTP. Moreover, examination of the time course for 6-deoxy-L-talan production proved that this system ran satisfactorily. This paper is the first report of a convenient method to readily produce the exopolysaccharide from A. actinomycetemcomitans in vitro.

摘要

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