Suzuki N, Nakano Y, Yoshida Y, Nakao H, Yamashita Y, Koga T
Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan.
Biochim Biophys Acta. 2000 Dec 15;1517(1):135-8. doi: 10.1016/s0167-4781(00)00229-3.
The serotype a-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans consists of 6-deoxy-D-talose. A gene cluster associated with the biosynthesis of SPA was cloned and sequenced from the chromosomal DNA of A. actinomycetemcomitans SUNYaB 75 (serotype a). This cluster consisted of 14 open reading frames. Insertional inactivation of eight genes in this cluster resulted in loss of the ability of A. actinomycetemcomitans SUNYaB 75 cells to produce the polysaccharide. A protein database search revealed that the 11 sequential genes containing these eight genes were not found in SPA-associated gene clusters of the other serotypes of A. actinomycetemcomitans. These results suggest that the gene cluster is unique to serotype a and is essential to the synthesis of the SPA.
伴放线放线杆菌a血清型特异性多糖抗原（SPA）由6-脱氧-D-塔罗糖组成。从伴放线放线杆菌SUNYaB 75（a血清型）的染色体DNA中克隆并测序了一个与SPA生物合成相关的基因簇。该基因簇由14个开放阅读框组成。该基因簇中8个基因的插入失活导致伴放线放线杆菌SUNYaB 75细胞失去产生多糖的能力。蛋白质数据库搜索显示，在伴放线放线杆菌其他血清型的SPA相关基因簇中未发现包含这8个基因的11个连续基因。这些结果表明，该基因簇是a血清型所特有的，对SPA的合成至关重要。
