Bernhardt Hannelore, Knoke M, Bernhardt J
Clinic of Internal Medicine A, University of Greifswald, Greifswald, Germany.
Mycoses. 2003;46(9-10):370-4. doi: 10.1046/j.0933-7407.2003.00908.x.
The aim was the investigation of fungal colonization and morphological alterations under the influence of voriconazole in an in vitro system. Voriconazole stopped growth and colonization of Candida albicans (wild type SC5314) on cover slips in microtiter plates dependent on drug concentration, the time of Candida growth before the input of voriconazole and oxygen concentration. The direct microscopy by fluorescence staining with the optical brightener Blancophor showed short bizarrely deformed mycelia looking swollen. The colonization on cover glass was diminished. Microcolonies or starting of biofilm formation as in the control was not observed. The metabolic activity was demonstrated by vital staining with FUN 1 resulting in red fluorescent structures in the yeast forms and mycelia in the controls. Under voriconazole influence the remaining cells only showed a green or pale yellow fluorescence. Most of the cells lost their metabolic activity.
目的是在体外系统中研究伏立康唑影响下的真菌定植和形态学改变。伏立康唑可抑制白色念珠菌（野生型SC5314）在微量滴定板盖玻片上的生长和定植，其效果取决于药物浓度、加入伏立康唑前念珠菌的生长时间以及氧浓度。用荧光增白剂布兰可佛（Blancophor）进行荧光染色的直接显微镜检查显示，菌丝体短而畸形，看起来肿胀。盖玻片上的定植减少。未观察到如对照组中那样的微菌落或生物膜形成起始。用FUN 1进行活细胞染色证明了代谢活性，对照组中酵母形式和菌丝体呈现红色荧光结构。在伏立康唑影响下，剩余细胞仅呈现绿色或浅黄色荧光。大多数细胞失去了代谢活性。
