自然感染猫中猫冠状病毒（FCoVs）的准种组成及系统发育分析
Quasispecies composition and phylogenetic analysis of feline coronaviruses (FCoVs) in naturally infected cats.
作者信息
Battilani Mara, Coradin Tiziana, Scagliarini Alessandra, Ciulli Sara, Ostanello Fabio, Prosperi Santino, Morganti Luigi
机构信息
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale-Servizio MIPAV, Alma Mater Studiorum, University of Bologna, Via Tolara di Sopra, 50, 40064 (Bo), Ozzano Emilia, Italy.
出版信息
FEMS Immunol Med Microbiol. 2003 Nov 28;39(2):141-7. doi: 10.1016/S0928-8244(03)00237-2.
Quasispecies composition and tissue distribution of feline coronaviruses (FCoVs) were studied in naturally infected cats. The genomic complexity of FCoVs was investigated using single-strand conformational polymorphism (SSCP) analysis of N and ORF7b amplicons, and the evolutionary process was investigated by sequence-based phylogenetic analysis. SSCP analysis showed high heterogeneity of the FCoV genome which was correlated with the seriousness of the clinical form. The two genomic regions analysed showed different levels of variation; the N region demonstrated significant heterogeneity as compared to ORF7b. Phylogenetic analysis of the nucleotide sequences showed the clear separation of sequences analysed on the basis of virulence and geographical origin. A maximum likelihood analysis of N and ORF7b data sets showed a situation of strong heterogeneity for the N region.
在自然感染的猫中研究了猫冠状病毒（FCoV）的准种组成和组织分布。使用N和ORF7b扩增子的单链构象多态性（SSCP）分析研究了FCoV的基因组复杂性，并通过基于序列的系统发育分析研究了进化过程。SSCP分析显示FCoV基因组具有高度异质性，这与临床症状的严重程度相关。所分析的两个基因组区域显示出不同程度的变异；与ORF7b相比，N区域表现出显著的异质性。核苷酸序列的系统发育分析表明，根据毒力和地理来源分析的序列明显分开。对N和ORF7b数据集的最大似然分析显示N区域存在强烈的异质性情况。
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