由过量产生球形红杆菌δ-氨基乙酰丙酸合酶的大肠杆菌δ-氨基乙酰丙酸脱水酶突变体生产δ-氨基乙酰丙酸。
Production of 5-aminolevulinic acid by an Escherichia coli aminolevulinate dehydratase mutant that overproduces Rhodobacter sphaeroides aminolevulinate synthase.
作者信息
Xie L, Eiteman M A, Altman E
机构信息
Center for Molecular BioEngineering, Driftmier Engineering, University of Georgia, Athens, GA 30602, USA.
出版信息
Biotechnol Lett. 2003 Oct;25(20):1751-5. doi: 10.1023/a:1026035912038.
Two Escherichia coli strains, a wild-type strain and a hemB mutant, which contained the hemA gene from Rhodobacter sphaeroides produced aminolevulinate at 4 g l(-1). The hemB mutant did not increase the accumulation of aminolevulinate, an observation attributed to its 50% lower aminolevulinate synthase activity in than the wild-type. Growth was limited for both strains probably due to the accumulation of 0.5 g aminoacetone l(-1).
两种大肠杆菌菌株，一种野生型菌株和一种hemB突变体，它们含有来自球形红细菌的hemA基因，能产生4 g l⁻¹的氨基乙酰丙酸。hemB突变体并未增加氨基乙酰丙酸的积累，这一观察结果归因于其氨基乙酰丙酸合酶活性比野生型低50%。两种菌株的生长可能都受到限制，这可能是由于积累了0.5 g l⁻¹的氨基丙酮。
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