Xie L, Eiteman M A, Altman E
Center for Molecular BioEngineering, Driftmier Engineering, University of Georgia, Athens, GA 30602, USA.
Biotechnol Lett. 2003 Oct;25(20):1751-5. doi: 10.1023/a:1026035912038.
Two Escherichia coli strains, a wild-type strain and a hemB mutant, which contained the hemA gene from Rhodobacter sphaeroides produced aminolevulinate at 4 g l(-1). The hemB mutant did not increase the accumulation of aminolevulinate, an observation attributed to its 50% lower aminolevulinate synthase activity in than the wild-type. Growth was limited for both strains probably due to the accumulation of 0.5 g aminoacetone l(-1).
两种大肠杆菌菌株，一种野生型菌株和一种hemB突变体，它们含有来自球形红细菌的hemA基因，能产生4 g l⁻¹的氨基乙酰丙酸。hemB突变体并未增加氨基乙酰丙酸的积累，这一观察结果归因于其氨基乙酰丙酸合酶活性比野生型低50%。两种菌株的生长可能都受到限制，这可能是由于积累了0.5 g l⁻¹的氨基丙酮。
