Kang Zhen, Wang Yang, Wang Qiang, Qi Qingsheng
State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.
Bioeng Bugs. 2011 Nov-Dec;2(6):342-5. doi: 10.4161/bbug.2.6.17237. Epub 2011 Nov 1.
5-Aminolevulinic acid (ALA) has recently attracted significant attentions due to its potential applications in many diverse fields. The majority of engineered ALA producers are based on the whole cell catalysis, supplemented with succinate and glycine as precursors. Recently, we succeeded in producing ALA directly from inexpensive glucose, through re-constructing the native C5 pathway of ALA synthesis in Escherichia coli. Herein, we further discuss ALA production by manipulating the C5 and C4 pathways in Escherichia coli through the strategy of metabolic engineering.
5-氨基乙酰丙酸（ALA）因其在众多不同领域的潜在应用，近来备受关注。大多数工程化的ALA生产者基于全细胞催化，以琥珀酸和甘氨酸作为前体进行补充。最近，我们通过在大肠杆菌中重建天然的ALA合成C5途径，成功地从廉价的葡萄糖直接生产出ALA。在此，我们进一步讨论通过代谢工程策略操纵大肠杆菌中的C5和C4途径来生产ALA。
