Chediac-Tannoury Rita, Araj George F
American University of Beirut Medical Center, Beirut, Lebanon.
Clin Lab Sci. 2003 Fall;16(4):198-202.
Methicillin resistant strains of Staphylococcus aureus (MRSA) are implicated in serious infections and nosocomial outbreaks, and show resistance to a wide range of antibiotics, thus limiting the treatment options. Therefore, rapid detection is clinically crucial for both treatment and infection control measures. This study assessed the performance of a rapid latex agglutination kit marketed to detect MRSA clinical isolates (MRSA-Screen test Denka Seiken Co Ltd, Tokyo, Japan) based on detecting a specific penicillin binding protein 2a (PBP2a) in comparison to the NCCLS oxacillin salt agar screen plate, the 1 microg oxacillin disk diffusion test, and the oxacillin MIC by E-test. Testing was carried out on 133 isolates consisting of 99 MRSA and 34 methicillin sensitive strains of S. aureus (MSSA). Concordant results were observed between the latex kit and all the other tests for the 99 MRSA isolates. Only 1 of the 34 MSSA isolates gave a positive agglutination reaction in the latex kit. The kit sensitivity and specificity were determined to be 100% and 97%, respectively. This reliable performance indicates that the MRSA-Screen latex test is very useful test for the rapid detection of MRSA isolates in the clinical microbiology laboratory.
耐甲氧西林金黄色葡萄球菌（MRSA）与严重感染及医院内感染暴发有关，并且对多种抗生素耐药，从而限制了治疗选择。因此，快速检测对于治疗和感染控制措施在临床上至关重要。本研究评估了一种用于检测MRSA临床分离株的快速乳胶凝集试剂盒（MRSA-Screen检测试剂盒，日本东京Denka Seiken有限公司）的性能，该试剂盒通过检测特定的青霉素结合蛋白2a（PBP2a），并与美国国家临床实验室标准委员会（NCCLS）的苯唑西林盐琼脂筛选平板、1μg苯唑西林纸片扩散试验以及E-test法测定的苯唑西林最低抑菌浓度（MIC）进行比较。对133株分离株进行了检测，其中包括99株MRSA和34株甲氧西林敏感金黄色葡萄球菌（MSSA）。对于99株MRSA分离株，乳胶试剂盒与所有其他检测方法的结果一致。34株MSSA分离株中只有1株在乳胶试剂盒中出现阳性凝集反应。该试剂盒的灵敏度和特异性分别确定为100%和97%。这种可靠的性能表明，MRSA-Screen乳胶试验对于临床微生物实验室快速检测MRSA分离株是一种非常有用的检测方法。
