Cavassini M, Wenger A, Jaton K, Blanc D S, Bille J
Institut de Microbiologie, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland.
J Clin Microbiol. 1999 May;37(5):1591-4. doi: 10.1128/JCM.37.5.1591-1594.1999.
The MRSA-Screen test (Denka Seiken Co., Ltd., Tokyo, Japan), consisting of a slide latex agglutination kit that detects PBP 2a with a monoclonal antibody, was blindly compared to the oxacillin disk diffusion test, the oxacillin-salt agar screen, and PCR of the mecA gene for the detection of methicillin resistance in Staphylococcus aureus. A total of 120 methicillin-susceptible S. aureus (MSSA) and 80 methicillin-resistant S. aureus (MRSA) isolates, defined by the absence or presence of the mecA gene, respectively, were tested. The MRSA-Screen test, the oxacillin disk diffusion test, and the oxacillin-salt agar screening test showed sensitivities of 100, 61.3, and 82.5% and specificities of 99.2, 96.7, and 98.3%, respectively. We conclude that the MRSA-Screen is a very accurate, reliable, and fast test (15 min) for differentiation of MRSA from MSSA colonies on agar plates.
采用MRSA-Screen检测试剂盒（日本东京电和化成工业株式会社）对金黄色葡萄球菌进行耐甲氧西林检测，该试剂盒为玻片乳胶凝集试验，采用单克隆抗体检测PBP 2a。将其与苯唑西林纸片扩散法、苯唑西林高盐琼脂筛选法以及检测mecA基因的聚合酶链反应（PCR）法进行盲法比较，以检测金黄色葡萄球菌的耐甲氧西林情况。共检测了120株耐甲氧西林金黄色葡萄球菌（MSSA）和80株耐甲氧西林金黄色葡萄球菌（MRSA），分别根据mecA基因的有无进行定义。MRSA-Screen检测法、苯唑西林纸片扩散法和苯唑西林高盐琼脂筛选试验的灵敏度分别为100%、61.3%和82.5%，特异性分别为99.2%、96.7%和98.3%。我们得出结论，MRSA-Screen是一种非常准确、可靠且快速（15分钟）的检测方法，可用于在琼脂平板上区分MRSA和MSSA菌落。
