Flores-Crespo J, Herrera-Rodríguez D, Mendoza de Gives P, Liébano-Hernández E, Vázquez-Prats V M, López-Arellano M E
Proyecto Control Biológico, Centro Nacional de Investigaciones Disciplinarias en Parasitología Veterinaria INIFAP, Estado de Morelos, México.
J Helminthol. 2003 Dec;77(4):297-303. doi: 10.1079/joh2003197.
The effect of oral administration of three different nematode-trapping fungi, in aqueous suspension containing either Dactylaria sp. or Arthrobotrys oligospora conidia or Duddingtonia flagrans chlamydospores, on the number of Haemonchus contortus infective larvae in sheep faeces, was evaluated. The three selected species of fungi produce three-dimensional adhesive nets in the presence of nematodes. Sixteen Creole sheep were divided into four groups of four animals each. Groups 1 and 2 were orally drenched with a suspension containing 2x10(7) conidia of either A. oligospora or Dactylaria sp. Group 3, received a similar treatment, with D. flagrans chlamydospores, instead of conidia, being administered, at the same dose. Group 4 acted as control, without any fungi. Faecal samples were collected directly from the rectum of each sheep and faecal cultures were prepared and incubated at 15 and 21 days. Larvae were recovered from faecal cultures and counted. The highest reduction of the nematode population occurred in the D. flagrans group, reaching reductions of 96.3% and 91.4% in individual samplings in plates incubated for 15 and 21 days, respectively. Arthrobotrys oligospora showed moderate reductions in the faecal larval population, ranging between 25-64% at 15 days incubation. In general, Dactylaria sp., was less efficient in its trapping ability. Despite the inconsistent results with Dactylaria sp., reduction percentages of 73.4% and 80.7% were recorded in individual samplings during the first and second days, in plates incubated for 15 days. Duddingtonia flagrans, was shown to be a potential biological control agent of H. contortus infective larvae.
评估了口服三种不同的捕食线虫真菌（其水悬浮液中含有指状节丛孢菌或少孢节丛孢分生孢子或达丁顿链霉菌厚垣孢子）对绵羊粪便中捻转血矛线虫感染性幼虫数量的影响。所选的这三种真菌在有线虫存在时会产生三维粘性网。16只克里奥尔羊被分成四组，每组四只动物。第1组和第2组口服含有2×10⁷少孢节丛孢菌或指状节丛孢菌分生孢子的悬浮液。第3组接受类似处理，用相同剂量的达丁顿链霉菌厚垣孢子代替分生孢子进行给药。第4组作为对照，不给予任何真菌。直接从每只羊的直肠采集粪便样本，制备粪便培养物并在15天和21天进行培养。从粪便培养物中回收幼虫并计数。线虫数量减少最多的是达丁顿链霉菌组，在培养15天和21天的平板中的单个样本中，分别减少了96.3%和91.4%。少孢节丛孢菌使粪便中的幼虫数量有中度减少，在培养15天时减少幅度在25% - 64%之间。总体而言，指状节丛孢菌的捕食能力较低。尽管指状节丛孢菌的结果不一致，但在培养15天的平板中，第一天和第二天的单个样本中分别记录到减少率为73.4%和80.7%。达丁顿链霉菌被证明是捻转血矛线虫感染性幼虫的一种潜在生物防治剂。
