Hao Min-qi, Xu Jun, Zhong Nan-shan
Guangzhou Institute of Respiratory Diseases, Guangzhou 510120.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2003;21(3):160-3.
To clone and analyze the cDNA of major allergen Der f 2 of Dermatophagoides farinae in south China.
cDNA of Der f 2 was cloned by RT-PCR, screened and their sequences were analyzed.
cDNA of Der f 2 was cloned. The sequence of the cloned Der f 2 was different with that published (D10448) in GenBank, with 87 additional nucleotides inserted into the 62th nucleotide of the original one. According to the original ORF, the deduced amino acids that were located prior or after the inserted 29 amino acid sequence showed no changes.
The cDNA of Der f 2 was cloned from Dermatophagoides farinae and its sequence showed significant difference with that reported in the GenBank.
克隆并分析中国南方地区粉尘螨主要变应原Der f 2的cDNA。
采用逆转录聚合酶链反应（RT-PCR）克隆Der f 2的cDNA，进行筛选并分析其序列。
成功克隆出Der f 2的cDNA。克隆的Der f 2序列与GenBank中公布的序列（D10448）不同，在原序列第62个核苷酸处插入了87个额外核苷酸。根据原始开放阅读框，插入的29个氨基酸序列前后推导的氨基酸无变化。
从粉尘螨中克隆出Der f 2的cDNA，其序列与GenBank报道的序列存在显著差异。
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