Tao Jun, Cai Wei-min, Chen Jia-lin, Zhang Yan-ping, Weng Hong-lei, Liu Rong-hua
Institute of Infectious Diseases, First Affiliatred Hospital, School of Medicine, Zhejiang University, Hangzhou 310003.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2003;21(3):173-5.
To explore the change of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9 (MMP-9) expression in liver tissue of the patients with schistosomiasis japonica.
Liver biopsy materials were examined pathomorphologically in 26 patients with schistosomiasis in advanced stage and 5 cases without the disease as control. The expression of MMP-2, MMP-9 and C-IV were studied by immunohistochemistry, and result was analyzed by picture quantitative analysis technique.
Immnoreactive MMP-2 was mainly expressed in the plasma, the membrane of hepatocytes and hepatic sinusoids, it was also found in myofibroblast cells and endothelial cells of blood vessel. Immnoreactive MMP-9 was observed in hepatic stellate cells, sinusoids and myofibroblast cells, sometimes it was also seen in the plasma of hepatocytes and epithelial cells of bile duct. The expression of MMP-2, MMP-9 and C-IV was significantly stronger in patients with advanced schistosomiasis than that of the control(P < 0.05). The expression of MMP-2 was relevant to the inflammation grade and stage of liver fibrosis(P < 0.01). The expression of MMP-9 did not show significant change(P > 0.05) and the expression of C-IV was consistent with that of MMP-2.
The study indicates that increased expression of MMP-2 participated in the initiation and development process of liver fibrosis, MMP-9 was related to the initiation of liver fibrosis and pathological change existed in hepatic sinusoids in advanced schistosomiasis japonica.
探讨日本血吸虫病患者肝组织中基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）表达的变化。
对26例晚期血吸虫病患者的肝活检材料进行病理形态学检查，并以5例非血吸虫病患者作为对照。采用免疫组织化学法研究MMP-2、MMP-9和Ⅳ型胶原（C-IV）的表达，并通过图像定量分析技术进行结果分析。
免疫反应性MMP-2主要表达于血浆、肝细胞膜及肝血窦，在肌成纤维细胞和血管内皮细胞中也有表达。免疫反应性MMP-9可见于肝星状细胞、血窦及肌成纤维细胞，有时也可见于肝细胞血浆和胆管上皮细胞。晚期血吸虫病患者MMP-2、MMP-9和C-IV的表达明显强于对照组（P < 0.05）。MMP-2的表达与肝纤维化的炎症分级和分期相关（P < 0.01）。MMP-9的表达无明显变化（P > 0.05），C-IV的表达与MMP-2一致。
研究表明，MMP-2表达增加参与了肝纤维化的起始和发展过程，MMP-9与肝纤维化的起始有关，晚期日本血吸虫病患者肝血窦存在病理改变。
