[Effects of catecholamines on human preadipocyte proliferation and differentiation].
作者信息
Zhu Xiao-hai, He Qing-lian, Lin Zi-hao
机构信息
Plastic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
出版信息
Zhonghua Zheng Xing Wai Ke Za Zhi. 2003 Jul;19(4):282-4.
OBJECTIVE
To study the effects of catecholamines on human preadipocyte proliferation and differentiation.
METHODS
Catecholamines (epinephrine, isoprenaline and noradrenaline) were added to the culture media of human preadipocytes. The proliferation of cells, the expression of GPDH and lipid droplet accumulation in cytoplasm were observed and recorded. The functions of alpha and beta receptors were examined with adding alpha and beta receptor blockers.
RESULTS
Epinephrine and isoprenaline stimulated human preadipocyte proliferation and inhibited GPDH up-regulation during differentiation. The three types of catecholamines inhibited lipid accumulation in cell differentiation. The beta-adrenoceptors played a key role during the process.
CONCLUSION
Human preadipocytes responded to catecholamine characteristically. The result would be applicable in the study of drugs for obesity.
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