[雄激素受体亚型在正常人类前列腺中的表达]
[Expression of androgen receptor isoforms in normal human prostate].
作者信息
Xia Shu-Jie, Hao Gang-Yue, Wen Wei, Zhang Jie, Li Wei-Guo, Zhu Yi-Yong, Tang Xiao-Da
机构信息
Department of Urology, Shanghai First People's Hospital, Jiaotong University, Shanghai 200080, China.
出版信息
Zhonghua Nan Ke Xue. 2003 Oct;9(7):501-3, 506.
OBJECTIVE
To investigate the expression of androgen receptor (AR) isoforms in normal human prostate.
METHODS
Fourteen normal prostatic specimens from donors, aged 25 on average (21-28 yr), were analyzed by high resolution isoelectric focusing (IEF). The expression of AR isoforms was demonstrated in all 14 normal human prostatic tissues.
RESULTS
Four types of AR isoforms were detected with isoelectric point value at 6.5, 6.0, 5.8 and 5.3 in 14 prostatic specimens. Binding of 3H-dihydrotestosterone (DHT) to these four AR isoforms was inhibited by the addition of 100-fold excess of DHT and testosterone. No effect of progesterone, estradiol and diethylstilbestrol on tritiated hormone binding was observed.
CONCLUSIONS
There are four AR isoforms in normal human prostate. The expression of AR isoforms is different from one another.
目的
研究雄激素受体（AR）异构体在正常人体前列腺中的表达。
方法
对14例平均年龄25岁（21 - 28岁）供体的正常前列腺标本进行高分辨率等电聚焦（IEF）分析。在所有14例正常人体前列腺组织中均证实了AR异构体的表达。
结果
在14例前列腺标本中检测到四种类型的AR异构体，其等电点值分别为6.5、6.0、5.8和5.3。加入100倍过量的双氢睾酮（DHT）和睾酮可抑制3H - 双氢睾酮与这四种AR异构体的结合。未观察到孕酮、雌二醇和己烯雌酚对氚标记激素结合的影响。
结论
正常人体前列腺中存在四种AR异构体。AR异构体的表达各不相同。
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