Xia S, Wang Y, Tang X
Department of Urology, Shanghai First People's Hospital, Shanghai 200080, China.
Zhonghua Yi Xue Za Zhi. 2001 Dec 25;81(24):1500-3.
To investigate the androgen receptor isoforms expression and its novel eDNA cloning in prostate tissue.
The tissue from a case of benign prostatic hyperplasia (BPH) was analyzed by high resolution isoelectric focusing (IEF) technique and molecular biological technique of cDNA cloning was used to determine its AR isoforms expression and genes of AR isoforms.
From the prostatic specimen, three types of AR isoforms were detected with pI values of 6.5, 6.0 and 5.3 respectively. A novel AR subtype cDNA cloning, named as PHAR-1 and including 1 913 nucleotides was isolated from the same BPH tissue. The GenBank accession number for the nucleotide sequence of this AR cDNA was BankIT372205 AF324243. The comparison between PHAR-1 and AR nucleotide sequence registered in GenBank showed that 961 nucleotides in three part of nucleotide sequence were the same, while the other 952 nucleotides were different.
These three AR isoforms may originate from the same gene. The results may be important for the study of BPH, or prostatic growth. It is also valuable in the field of pharmacotherapy for treatment of BPH and prostatic cancer.
研究前列腺组织中雄激素受体异构体的表达及其新的 cDNA 克隆。
采用高分辨率等电聚焦（IEF）技术分析 1 例良性前列腺增生（BPH）患者的组织，并运用 cDNA 克隆的分子生物学技术来确定其雄激素受体异构体的表达及基因。
在前列腺标本中检测到三种类型的雄激素受体异构体，其等电点（pI）值分别为 6.5、6.0 和 5.3。从同一 BPH 组织中分离出一种新的雄激素受体亚型 cDNA 克隆，命名为 PHAR - 1，包含 1913 个核苷酸。该雄激素受体 cDNA 的核苷酸序列在 GenBank 中的登录号为 BankIt372205 AF324243。将 PHAR - 1 与 GenBank 中登记的雄激素受体核苷酸序列进行比较，结果显示核苷酸序列的三个部分中有 961 个核苷酸相同，而另外 952 个核苷酸不同。
这三种雄激素受体异构体可能源自同一基因。这些结果可能对 BPH 或前列腺生长的研究具有重要意义。在 BPH 和前列腺癌的药物治疗领域也具有重要价值。
