Ueda Yoko, Hagihara Masao, Okamoto Ayako, Higuchi Ayako, Tanabe Akiko, Hirabayashi Kaori, Izumi Shunichiro, Makino Tsunehisa, Kato Shunichi, Hotta Tomomitsu
Department of Hematology and Oncology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa, Japan.
Hum Immunol. 2003 Dec;64(12):1144-51. doi: 10.1016/j.humimm.2003.08.342.
CD11c(+) peripheral blood myeloid dendritic cells (MDC), and CD123(bright) plasmacytoid cells (PDC), are two human dendritic cell (DC) populations. In the present study, the percentages of MDC [lin(-)/HLA-DR(+)/CD123(-)/CD11c(+)] and PDC [lin(-)/HLA-DR(+)/CD123(bright)] in a peripheral blood (PB) sample obtained from women at the time of delivery and in their umbilical cord blood (UCB) were compared with those in the PB of healthy volunteers. The percentages of MDC and PDC were determined by four-color flow cytometry. The percentages of MDC and PDC were significantly lower in the PB obtained at delivery (MDC, 0.11% +/- 0.08%; PDC, 0.06% +/- 0.06%) and in the UCB (MDC, 0.15% +/- 0.03%; PDC, 0.05% +/- 0.05%) than in the PB of the controls (males, 0.28% +/- 0.12%, 0.11% +/- 0.06%; females, 0.32% +/- 0.13%, 0.11% +/- 0.07%). Among the pregnant women at delivery, there were significant correlations between the percentage of MDC or percentage of PDC in the PB at delivery and the respective parameter in the UCB. The PB of other pregnant women, at 20-35 weeks of gestational age, also indicated significantly low percentages of MDC and PDC (0.1% +/- 0.1%, 0.06% +/- 0.07%). The ratio of MDC/PDC was not reduced in the UCB (4.25 +/- 2.74), but was reduced in the PB obtained at delivery and the PB obtained during pregnancy (0.79 +/- 0.91) in comparison with that in age-matched, nonpregnant females (3.96 +/- 2.95). It was assumed that reduction of MDC during pregnancy plays an important role in maintaining immune tolerance against the embryo.
CD11c(+)外周血髓样树突状细胞（MDC）和CD123(明亮)浆细胞样细胞（PDC）是两个人类树突状细胞（DC）群体。在本研究中，将分娩时从女性获取的外周血（PB）样本及其脐带血（UCB）中MDC [lin(-)/HLA-DR(+)/CD123(-)/CD11c(+)]和PDC [lin(-)/HLA-DR(+)/CD123(明亮)]的百分比与健康志愿者外周血中的相应百分比进行比较。通过四色流式细胞术测定MDC和PDC的百分比。分娩时获取的PB（MDC，0.11%±0.08%；PDC，0.06%±0.06%）和UCB（MDC，0.15%±0.03%；PDC，0.05%±0.05%）中MDC和PDC的百分比显著低于对照组的PB（男性，0.28%±0.12%，0.11%±0.06%；女性，0.32%±0.13%，0.11%±0.07%）。在分娩时的孕妇中，分娩时PB中MDC百分比或PDC百分比与UCB中的相应参数之间存在显著相关性。其他孕周为20 - 35周的孕妇的PB也显示MDC和PDC的百分比显著降低（0.1%±0.1%，0.06%±0.07%）。与年龄匹配的未孕女性相比，UCB中MDC/PDC的比例未降低（4.25±2.74），但分娩时获取的PB和孕期获取的PB中该比例降低（0.79±0.91）。据推测，孕期MDC的减少在维持对胚胎的免疫耐受中起重要作用。
