Lin Hai-Rong, Wang Ya-Zhe, Wang Dong-Xia, Chang Yan, Hao Le, Qin Ya-Zhen, Li Jin-Lan, Li Ling-Di, Huang Xiao-Jun, Liu Yan-Rong
Institute of Hematology, People Hospital, Peking University, Beijing 100044, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2009 Oct;17(5):1249-54.
In order to study the quantity of dendritic cell (DC) subsets of bone marrow in patients with acute myeloid leukemia (AML), the bone marrow aspirate were collected from 77 newly diagnosed AML patients and from 30 healthy persons. The quantity of DC subsets (myeloid dendritic cells, mDC and plasmacytoid dendritic cells, pDC) were detected by flow cytometry and analysed by 3-color and 4-color cytometric gate. Based on the conventional 3-color panel, mDC were identified by Lin-HLA-DR+CD11c+ and pDC were identified by Lin-HLA-DR+CD123+. Based on the 4-color panel, mDC were identified by Lin-HLA-DR+CD11c+ BDCA-1+ and pDC were identified by Lin-HLA-DR+CD123+BDCA-2+. The results showed that a reduction of mDC was found in 74.0% (57/77) and 58.4% (45/77) patients, a reduction of pDC was found in 90.9% (70/77) and 46.8% (36/77) patients respectively by 3-color and 4-color cytometric analysis. Meanwhile an expansion of mDC was showed in 19.5% (15/77) and 22.1% (17/77) patients, an expansion of pDC was showed in 1.3% (1/77) and 27.3% (21/77) patients respectively by 3-color and 4-color cytometric analysis. In subtypes of AML-M2, AML-M3 or AML-M4/5, 81.4%, 100% and 42.1% patients showed mDC decrease and 88.4%, 100% and 89.5% patients showed pDC decrease respectively by 4-color cytometric analysis. It is concluded that the 4-color cytometric gate is better method for detection of mDC and pDC from bone marrow of newly diagnosed AML patients as compared with 3-color cytometric gate, the majority of AML patients showed reduction of mDC and pDC. The percentages of patients with mDC normal or mDC increase in AML-M4/5 subtypes are more than that in AML-M2/3 subtypes, while the pDC does not show difference between AML subtypes.
为研究急性髓系白血病（AML）患者骨髓中树突状细胞（DC）亚群的数量，收集了77例新诊断的AML患者及30例健康人的骨髓穿刺液。采用流式细胞术检测DC亚群（髓样树突状细胞，mDC和浆细胞样树突状细胞，pDC）数量，并通过三色和四色细胞术设门进行分析。基于传统三色组合，mDC通过Lin-HLA-DR+CD11c+鉴定，pDC通过Lin-HLA-DR+CD123+鉴定。基于四色组合，mDC通过Lin-HLA-DR+CD11c+BDCA-1+鉴定，pDC通过Lin-HLA-DR+CD123+BDCA-2+鉴定。结果显示，通过三色和四色细胞术分析，分别有74.0%（57/77）和58.4%（45/77）的患者mDC减少，90.9%（70/77）和46.8%（36/77）的患者pDC减少。同时，通过三色和四色细胞术分析，分别有19.5%（15/77）和22.1%（17/77）的患者mDC增多，1.3%（1/77）和27.3%（21/77）的患者pDC增多。在AML-M2、AML-M3或AML-M4/5亚型中，通过四色细胞术分析，分别有81.4%、100%和42.1%的患者mDC减少，88.4%、100%和89.5%的患者pDC减少。结论：与三色细胞术设门相比，四色细胞术设门是检测新诊断AML患者骨髓中mDC和pDC的更好方法，大多数AML患者mDC和pDC减少。AML-M4/5亚型中mDC正常或增多的患者百分比高于AML-M2/3亚型，而各AML亚型间pDC无差异。
